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The influence of amifostine administration prior to cyclophosphamide on in vitro maturation of mouse oocytes |
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| Authors: | Afsaneh Golkar-Narenji Zeinab Barekati Hussein Eimani Fatemeh Shabani Hamid Gourabi |
| Institution: | 1. Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, No 12, Hafez, Banihashem St. Resalat Ave, Tehran, 19295-4644, Iran 2. Laboratory for Gynecological Oncology, Women’s Hospital/Department of Biomedicine, University of Basel, Hebelstrasse 20, 4031, Basel, Switzerland 3. Department of Embryology at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran 4. Department of Epidemology and Reproductive Health at Reproductive Epidemiology Research Center, Royan Institute For Reproductive Biomedicine, ACECR, Tehran, Iran |
| Abstract: | PurposeThe protective effect of amifostine against cyclophosphamide (CP) was evaluated on mouse oocytes.Materials and methodsFemale mice were divided into four groups as follows: group1: cyclophosphamide (CP) (75 mg/kg, i.p) injection, group2: amifostine (250 mg/kg, i.p) injection, group3: amifostine (250 mg/kg, i.p) administered prior to CP (75 mg/kg, i.p) injection, Control group with injection of saline. About 21 days after injection, in vitro maturation (IVM) of oocytes was recorded. Furthermore the percentage of aneuploid oocytes was determined.ResultsIn the CP group IVM rate was significantly decreased and aneploidy rate was significantly increased when compared to other groups (p?DiscussionDepletion in IVM rate with administration of CP is due its adverse effects on oocyte quality. Amifostine administration prior to CP injection appears to modulate deleterious effects of CP on oocytes. |
| Keywords: | Amifostine Aneuploidy Chromosome Cyclophosphamide Maturation Oocyte |
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