| The antiapoptotic effect of insulin against anoxia/reoxygenation injury in cultured cardiomyocyte of neonatal rat |
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| 作者姓名： | Xiang Gu Chunzhi Shi Yibai Feng Ming Li Zuolin Fu Xinping Zhang |
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| 作者单位： | [1]Institute of Cardiology, Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China |
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| 摘 要： | Objective： To study protective effect of insulin against cardiomyocyte apoptosis in anoxia/reoxygenation （A/R） injury of neonatal rat. Methods： The model of A/R injury was finished through receiving anoxia for 2h and reoxygenation for 4h in cultured cardiomyocytes of neonatal rat. The cardiomyocytes were divided randomly into 3 groups： control group （CON）, anoxia/reoxygenation group （A/R） and insulin-treated group （INS）. At the end of reoxygenation of 4 hours, activities of lactate dehydrogenase （LDH）, contents of malondiaidehyde （MDA）, were assessed through spectrophotometric procedures, myocyte apoptosis were detected through TUNEL and DNA Ladder. Results： MDA, LDH, and Apoptosis Index were significantly decreased in INS group compared with A/R group （P〈0.01）. Conclusion： Insulin has a protective effect against A/R injury in cultured cardiomyocyte of neonatal rat; the protective mechanism may contribute to antiapoptosis of insulin.
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| 关 键 词： | 胰岛素抵抗 缺氧再灌注损伤 保护机制 小鼠 动物实验 |
| 收稿时间： | 2005-05-10 |
The antiapoptotic effect of insulin against anoxia/reoxygenation injury in cultured cardiomyocyte of neonatal rat |
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| Xiang Gu,Chunzhi Shi,Yibai Feng,Ming Li,Zuolin Fu,Xinping Zhang.The antiapoptotic effect of insulin against anoxia/reoxygenation injury in cultured cardiomyocyte of neonatal rat[J].Acta Universitatis Medicinalis Nanjing,2005,19(5):232-235. |
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| Authors: | Xiang Gu Chunzhi Shi Yibai Feng Ming Li Zuolin Fu Xinping Zhang |
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| Abstract: | Objective: To study protective effect of insulin against cardiomyocyte apoptosis in anoxia/reoxygenation (A/R) injury of neonatal rat. Methods:The model of A/R injury was finished through receiving anoxia for 2 h and reoxygenation for 4 h in cultured cardiomyocytes of neonatal rat. The cardiomyocytes were divided randomly into 3 groups: control group (CON), anoxia/reoxygenation group (A/R) and insulin-treated group (INS). At the end of reoxygenation of 4 hours, activities of lactate dehydrogenase (LDH), contents of malondialdehyde (MDA) were assessed through spectrophotometric procedures, myocyte apoptosis were detected through TUNEL and DNA Ladder. Results: MDA, LDH, and Apoptosis Index were significantly decreased in INS group compared with A/R group (P<0.01). Conclusion: Insulin has a protective effect against A/R injury in cultured cardiomyocyte of neonatal rat; the protective mechanism may contribute to antiapoptosis of insulin. |
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| Keywords: | insulin cultured cardiomyocyte anoxia/reoxygenation apoptosis |
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