B细胞淋巴瘤的Fas蛋白表达及意义

目的：探讨Fas蛋白在不同类型B细胞源性非霍奇金淋巴瘤（non-Hodgkon‘s Bcell lymphoma,B-NHL)中的分布及意义，为B－NHL诊治提供新的观察指标及实验依据。方法：对确诊为B－NHL的79例及良性淋巴组织20例的石蜡切片，用免疫组化方法进行Fas蛋白表达的检测。结果：发现B－NHL的Fas蛋白平均阳性表达率为65．8％，良性淋巴组织为60．0％，单从数量上，良恶两者之间差异无显著性（P＜0．05），而从阳性细胞分布上良、恶性可见区别，良性分布有规律，主要集中在生发中心；恶性分布无规律性。淋巴瘤组内各亚组之间差异有显著性意义：弥漫大B细胞淋巴瘤（deffuse large B cell lymphoma,DLBL)组表达分别高于滤泡性淋巴瘤（follicular cell lymphoma,FCL)(P＜0．05）和小淋巴细胞性淋巴瘤（small lymphocytic lymphoma,SLL)组（P＜0．01）；FCL组表达高于SLL组（P＜0．05）。Fas蛋白的表达与性别、年龄及部位无相关性。结论：1、Fas的表达与B淋巴瘤的类型及恶性程度有关。2、Fas的表达半定量检测不能作为鉴别良恶性的指标，而分布规律不同可作参考。检测Fas蛋白的数量及注意阳性细胞分布规律，对病理确诊淋巴瘤疑难病例有一定应用价值，可为临床选择治疗方案提供参考。

Fas Protein Expression and Its Significance in Patients with Non-Hodgkins B Cell Lymphoma

Objective: The aim of this study was to investigate the distribution and the significance of Fas protein in subtypes of non-Hodgkin's B cell lymphoma (B-NHL), and to provide a new marker for observation and experimental evidence for diagnosis of the patients with B cell lymphoma. Methods: Fas protein expression in paraffin embedded sections of 79 cases conformed B-NHL and of 20 cases benign lymphoid tissue were determined by immunohistochemistry. Results: Fas protein expression was found in 65. 8% of B-NHL and 60. 0% of benign lymphoid tissue. There was no significant difference about quantity between B-NHL group and benign group(P > 0. 05 ), but positive location of Fas protein is different. There were significant differences among three subtypes of B-NHL (P < 0. 05). The expression of Fas protein in diffuse large B cell lymphoma (DLCL) was higher than that in follicular cell lymphoma(FCL) (P < 0. 05) and also than that in small lymphocytic lymphoma (SLL) (P < 0. 01 ). The expression of Fas protein in FCL was higher than that in SLL (P < 0. 05). No significant correlation was found among Fas expression, sex, age, and tumor location (P > 0. 05 ). Conclusions: There may be association in expression of Fas protein among subtypes and grades of B-NHL. The account of Fas expression is not useful for distinguishing benign from malignant groups, but it may be worthful to find locational characteristics of Fas protein in distinguishing benign and malignant lymphoid diseases. It should be valuable to detect quantity and distributuion of Fas protein in conform diagnosis of some difficult cases of B-NHL.