纳米级颅骨替代及诱导材料与颅骨成骨细胞的生物相容性体外实验研究

目的观察新型纳米级颅骨替代及诱导材料对体外培养的颅骨成骨细胞生长的影响，评价其生物相容性，为新型颅骨修复材料的临床应用提供依据。方法体外培养兔颅骨骨膜成骨细胞，将成骨细胞与纳米级颅骨替代材料及诱导材料一同培养。观察成骨细胞的形态学、生长情况及成骨能力变化。结果体外培养的兔颅骨骨膜源性细胞具有典型的成骨细胞形态，且具有体外成骨能力。与新型颅骨修复材料联合培养后细胞形态正常，生长繁殖良好，能够在材料表面成骨；与共培养前比较，细胞周期及生长曲线无显著性差异。结论纳米级颅骨替代及诱导材料具有良好的生物相容性。

Biocompatibility of the nanometer-scale substitute for cranial defect and osteoconductive materials with periosteal-derived osteoblasts in the calvaria: an in vitro experimental study

Objective To observe the effect of nanometer-scale substitute for cranial defect and osteoconductive materials on growth of periosteal-derived osteoblasts in the calvaria and evaluate the biocompatibility of the materials. Methods The cranial periosteal-derived osteoblasts were cultured by enzyme methods in vitro, and then the cultured cells were planted onto the nanometer-scale substitute and osteoconductive materials. The biological characteristics and osteogenic ability were observed. Results The cultured cells presented classical characteristics of osteoblasts and osteogenic ability, and the cells planted on the surface of the new-type cranial repairing materials showed the same characteristics. The apoptosis rate and MTT value show no significant difference between pre- and post-cocultures. Conclusion The new nanometer-scale substitute and osteoconductive material has good biocompatibility, and is applied to repair cranial defects.