N-V蛋白酶体内外纤维蛋白溶解活性的实验研究

目的研究NV蛋白酶对纤溶系统的影响。方法采用纤维蛋白平板法,观察NV蛋白酶体外溶解纤维蛋白的活性。通过一次静脉给药后不同时间测定家兔纤维蛋白原(Fg)、D二聚体(DDimer)的含量及血浆优球蛋白溶解时间(ELT),观察NV蛋白酶对正常家兔纤维蛋白溶解活性的影响。通过测定家兔凝血酶原时间(PT)、活化部分凝血活酶时间(APTT),观察NV蛋白酶对凝血功能的作用。结果NV蛋白酶具有体外溶解纤维蛋白的活性,测定效价为30000kU·g-1。NV蛋白酶(6000U·kg-1体重,15000U·kg-1体重)可明显减少DDimer、Fg含量,缩短ELT,对PT、APTT无明显影响。结论NV蛋白酶具有纤维蛋白溶解活性,对凝血功能未见有影响。

The experimental study on fibrinolytic activity of N-V proteinase in vivo and vitro

Aim To study the influence of N-V proteinase on fibrinolytic system. Methods The fibrin plate method was used to study the hydrolyzing fibrin activity of N-V proteinase in vitro. The content of Fg and D-Dimer, and ELT were assayed to study the effect of N-V proteinase on activity of fibrinolysis in normal rabbits. PT, APTT were measured to observe the effect of N-V proteinase on blood coagulation function in vivo. Results N-V proteinase possessed the fibrinolytic activity in vitro. The titer was 30 000 U·mg -1.N-V proteinase( 6 000 U·kg -1, 15 000 U·kg -1). It obviously decreased the content of Fg and D-Dimer, and lessened ELT, while had not influence the APTT and PT. Conclusion N-V proteinase possesses the fibrinolysis activity, and the effect on blood coagulation function are not observed.