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Isolation and characterization of species-specific microsatellite loci in African penguin (Spheniscus demersus)
Authors:Christiaan Labuschagne  Anna M. van Wyk  Antoinette Kotzé  Paul Grobler  Desiré L. Dalton
Affiliation:1. Genetics Department, University of the Free State, P.O. Box 339, Bloemfontein, 9300, South Africa
3. Inqaba Biotechnical Industries (Pty) Ltd, P.O. Box 14356, Hatfield, 0028, South Africa
2. National Zoological Gardens of South Africa, P.O. Box 754, Pretoria, 0001, South Africa
Abstract:Eight microsatellite markers were developed via pyrosequencing of a microsatellite-enriched library for the African Penguin (Spheniscus demersus). These microsatellite loci displayed 2–6 alleles with expected heterozygosity values ranging between 0.316 and 0.782 and observed heterozygosity between 0.381 and 0.84. These loci may be suitable for assessing patterns of genetic variability in African penguin. This is the first development of species-specific markers for the African penguin.
Keywords:
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