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Assignment test method using hypervariable markers for blue marlin (Makaira nigricans) stock identification |
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| Authors: | Laurie Sorenson Jan R. McDowell Trey Knott John E. Graves |
| Affiliation: | 1. College of William and Mary, Virginia Institute of Marine Science, PO Box 1346, Gloucester Point, VA, 23062, USA 3. Department of Ecology and Evolutionary Biology, University of California, Los Angeles, 610 Charles E Young Drive South, Terasaki Life Sciences Building #2154, Los Angeles, CA, 90095, USA 2. Center for Coastal Environmental Health and Biomolecular Research, National Ocean Service, 219 Ft. Johnson Road, Charleston, SC, 29412, USA |
| Abstract: | Blue marlin (Makaira nigricans) stock status varies among ocean basins, and the Atlantic-wide stock is overfished. United States regulations prohibit commercial landing, importation and sale of Atlantic blue marlin, but not of Pacific or Indian Ocean conspecifics. We genotyped 344 blue marlin of known origin and 16 samples used as unknowns at 13 microsatellite loci and the mitochondrial control region. Assignment tests were conducted using GENECLASS2 to investigate the efficacy of available genotypic data to identify individual origin. We successfully discriminated Atlantic and Pacific blue marlin using genetic characters, providing more power to assign marketed blue marlin products to ocean of origin. |
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