五氧化二砷体外对NB4细胞增殖及程序化死亡作用的研究

目的：探讨五氧化二砷（As2O3)对急性早幼粒白血病（APL）细胞NB4增殖及细胞程序化死亡的影响。方法：用细胞生长曲线，集落培养，流式细胞仪检测，DNA电泳，bcl-2蛋白和P53蛋白表达等方法观察NB4细胞增殖，凋亡和癌基因表达情况。结果：As2O5能显著地抑制NB4细胞增殖和细胞生长活力，诱导NB4细胞程序化死亡，下调p53,bcl-2蛋白表达，并能抑制造血祖细胞集落形成。结论：As2O5对APL细胞NB4具有较强的抑制和诱导凋亡作用，可能成为治疗APL有效的药物之一。

In vitro study on effect of As2O5 on NB4 cells proliferation and programmed cell death

Objective To study the effect of As2O5 on NB4 cell proliferation and programmed cell death. Methods NB4 cell proliferation, apoptosis and cancer gene expression were examined by cell growth curve, CFU-NB4 culture, flow cytometry, DNA electrophoresis, p53 and bcl-2 protein expression. Results As2O5 could significantly inhibit the proliferation of NB4 cell, induce programmed cell death and down-regulate p53, bcl-2 expression. Moreover, it could also inhibit CFU-GM and BFU-E in bone marrow cultures of normal human. Conclusion As2O5 could induce apoptosis and inhibit proliferation on acute promyelocytic leukemia(APL) cell line NB4. As2O5 may be one of effective medicines in the treatment of APL.