Esculetin modulates cytotoxicity induced by oxidants in NB4 human leukemia cells |
| |
Authors: | Virginia Rubio Ana Isabel García-Pérez Angel Herráez M. Cristina Tejedor José C. Diez |
| |
Affiliation: | Departamento de Biología de Sistemas, Unidad de Bioquímica y Biología Molecular, Campus Universitario, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain |
| |
Abstract: | Esculetin is a polyphenolic compound with cytoprotective properties. We previously demonstrated the induction of apoptosis by esculetin in NB4 human leukemia cells, as a model, by a mechanism not well understood. To analyse the antioxidant activity of esculetin on apoptosis, we have studied the influence of co-treatments of esculetin at a concentration of 100 μM with exogenous ROS donors, namely tert-butyl-hydroperoxide and hydrogen peroxide, on NB4 cells. Esculetin (100 μM) exerts a protective effect on cell viability and death necrosis or late apoptosis caused by the oxidant t-BHP whereas it potentiates decrease of cell viability and cell death caused by H2O2. In the first case, the O2? scavenging activity of esculetin (100 μM) could be implicated. In the last one, cytotoxicity by apoptosis induction seems to be related to the increase in O2?, among other possible mechanisms. These results contribute to the study of the antitumor properties of esculetin by regulation of redox balance in leukemia cells. |
| |
Keywords: | APL acute promyelocytic leukemia Annexin V-FITC fluorescein isothiocyanate coupled to annexin V BSO buthionine-[S, R]-sulfoximine DMSO dimethyl sulfoxide 2′,7′-dichlorodihydrofluorescein diacetate HEL human erythromegakaryocytic leukemia hydrogen peroxide DHE hydroethidine PI propidium iodide MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ROS reactive oxygen species Antioxidants Apoptosis Esculetin Hydrogen peroxide Reactive oxygen species Corresponding author. |
本文献已被 ScienceDirect 等数据库收录! |
|