Deep partial thickness burn blister fluid promotes neovascularization in the early stage of burn wound healing |
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| Authors: | Shin‐Chen Pan MD Li‐Wha Wu PhD Chung‐Lin Chen MD Shyh‐Jou Shieh MD PhD Haw‐Yen Chiu MD PhD |
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| Institution: | 1. Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan;2. Section of Plastic and Reconstructive Surgery, Department of Surgery, National Cheng Kung University Hospital, Tainan, Taiwan;3. Institute of Molecular Medicine and Cardiovascular Research Center, College of Medicine, National Cheng Kung University, Tainan, Taiwan |
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| Abstract: | The effect of burn blister fluid in neovascularization during burn wound healing is unknown. Burn blister fluid, containing a large amount of chemokines, is thought to play a role in the early stage of neovascularization. This process includes angiogenesis and vasculogenesis. Because of different healing time of burn wounds, we hypothesized that neovascularization in superficial partial thickness burn (SPTB) and deep partial thickness burn (DPTB) wounds were different. The neovasculogenic effects of two different burn blister fluids were also different. We found Day 7 DPTB wounds had a significant increase in blood vessels compared with SPTB wounds by immunohistochemistry. DPTB blister fluid significantly promoted neovascularization via increasing endothelial cell proliferation, and migration and differentiation of circulating angiogenic cells relative to SPTB blister fluids. In the animal study, DPTB blister fluids markedly promoted new blood vessel formation compared with those from SPTB blister fluids using in vivo Matrigel plug assay. These results suggest that DPTB wounds require more new vessel formation than SPTB. Furthermore, the measurement of angiogenic activities in burn blister fluids serves as a possible tool for assessing burn wound status. |
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