| Intralipid对吞噬细胞氧自由基产生的影响 |
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| 引用本文： | 吴国豪. Intralipid对吞噬细胞氧自由基产生的影响[J]. 肠外与肠内营养, 1999, 6(3): 138-140 |
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| 作者姓名： | 吴国豪 |
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| 作者单位： | 1. 上海医科大学附属中山医院普外科,上海,200032
2. Department of Clinical Bacteriology and Surgery, Huddinge University Hospital,Karolinska Institute,Sweden |
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| 摘 要： | 目的 观察Ｉｎｔｒａｌｉｐｉｄ对内毒素所致的多形核白细胞氧自由基产生的影响，方法 取２２例健康志愿者静脉血，采用Ｂｏｙｕｍ法提取多核白细胞（ＰＭＮ）并在体外培养，采用不同剂量的脂多糖（ＬＰＳ）刺激ＰＭＮ，并和不同剂量Ｉｎｔｒａｌｉｐｉｄ共同作用，采用ＮＢＴ还原剂试验测定氧自由基的产生量。结果 ＬＰＳ刺激各组的ＮＢＴ值明显高于非刺激组，而且ＮＢＴ值与ＬＰＳ浓度明显相关，Ｉｎｔｒａｌｉｐｉｄ可明显浓度
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| 关 键 词： | 吞噬细胞 氧自由基 |
| 修稿时间： | 1998-11-26 |
Intralipid decreases the release of oxygen radicals from phagocytes |
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| WU Guo-hao,Connie Jarstrand,Jorgen Nordenstrom. Intralipid decreases the release of oxygen radicals from phagocytes[J]. Parenteral & Enteral Nutrition, 1999, 6(3): 138-140 |
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| Authors: | WU Guo-hao Connie Jarstrand Jorgen Nordenstrom |
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| Abstract: | Objectives: To study the effect of Intralipid on the release of oxygen radicals from phagocytes in vitro. Methods: Human polymorphonuclear neutrophils(PMN) were purified and incubated either with purified lipopolysaccharides(LPS) or with Intralipid.The release of oxygen radicals was measured by the nitroblue tetrazolium(NBT) test. Results: The incubation of PMN with LPS increased the release of oxygen radicals.However,when the PMN were incubated with LPS together with Intralipid,the release of oxygen radicals was significantly decreased. Conclusions:Intralipid might inhibit the effect of LPS on the PMN in an infection with Gramnegtive bacteria. |
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| Keywords: | Intralipid |
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