端粒酶抑制剂对葡萄膜黑色素瘤细胞生长的抑制作用

目的 研究两种端粒酶抑制剂干扰素α 2b(IFN-α 2b)和顺铂(CDDP)对人葡萄膜黑色素瘤细胞生长抑制作用,为临床化疗人葡萄膜黑色素瘤提供更多依据.方法 用不同浓度及不同作用时间的IFN-α2b和CDDP作用于体外培养的人葡萄膜黑色素瘤细胞.倒置显微镜、电子显微镜观察其形态学变化,并用MTT法检测干扰剂对细胞生长的抑制作用.结果 随着IFN-α 2b和CDDP浓度的增加及作用时间的延长,细胞出现生长抑制的形态学变化,电子显微镜显示细胞出现凋亡;作用72 h,IFN-α 2b和CDDP浓度分别大于500IU/ml及1.00mg/L时细胞出现抑制状态,并在浓度分别达到5000IU/ml及10.00 mg/L时其细胞抑制率(66.1±4.7)%及(74.0±3.3)%开始与对照组出现差异有统计学意义(P<0.001);IFN-α 2b(5000 IU/ml)和CDDP(10.00 mg/L)浓度同定,作用时间达到48 h后细胞出现抑制状态,72 h后抑制率(67.9±8.1)%及(76.5±1.6)%开始与对照组出现差异有统计学意义(P<0.001);即随着两者浓度升高及作用时间增长,葡萄膜黑色素瘤细胞受到的抑制作用逐渐增强(P<0.01).结论 端粒酶抑制剂IFN-α2b和CDDP可有效抑制体外培养的人葡萄膜黑色素瘤细胞的生长,且随着两者浓度升高及作用时间延长抑制作用加强,可造成细胞凋亡.

Inhibitory effects of telomerase inhibitor to the growth of uveal melanoma cells

Objective To study the restrain effects of two telomerase inhibitors included IFN-α2b (interferon-α2b)and CDDP(cisplatin;cis-dichlorodiamine platinum)to uveal melanoma cells of human eyes,and to provide theory bases for chemotherary of uveal melanoma.Methods Applied IFN-α2b and CDDP to the primary cultured uveal melanoma cells of human eyes individually with different concentration and duration,and compared with control group.Morphological changes were observed by inverted optical and electronic microscope,and the restrain effects were detected by MTT.Results More and more morphological changes of restraint and apoptosis of cells were observed with the increasing concentration and duration of IFN-α 2b and CDDP.When action durations were 72 h.the cells were inhibited with the concentration more than 500 IU/ml(IFN-α 2b)and 1.00 mg/L(CDDP)respectively.The cells restrain rates were(66.1±4.7)%and(74.0+3.3)%with the concentration more than 5000 IU/ml(IFN-α2b)and 10 mg/L (CDDP),and the rates were higher than before(P<0.001).When concentrations were 5000 IU/ml(IFN-α2b)and 10.00 mg/L(CDDP),cells were inhibited at 48 h and the restrain rates were(67.9±8.1)%and(76.5±1.61%at 72 h,respectively,(P