经导管肝动脉化疗栓塞对肝癌患者外周血黑色素瘤抗原1 mRNA的影响

目的 探讨经导管肝动脉化疗栓塞(TACE)对肝癌患者外周血黑色素瘤抗原1(MAGE-1)mRNA表达水平的影响.方法 采用巢式RT-PCR方法 测定18例肝癌患者TACE治疗前后外周血MAGE-1 mRNA的表达.分析其与肝癌临床病理特征间的关系.结果 18例肝癌患者治疗前外周血MAGE-1 mRNA表达10例阳性、8例阴性;TACE治疗后11例阳性、7例阴性.治疗前后外周血MAGE-1mRNA阳性率差异无统计学意义(55.6%比61.1%.P>0.05).治疗前后肝癌患者外周血MAGE-1mRNA阳性率与肿瘤TNM分期((0%比76.9%,20%比76.9%,P<0.05)、远处转移有关(85.7%比36.4%,100%比36.4%,P<0.05),而与肿瘤大小、血清AFP无关(P>0.05).结论 TACE治疗肝癌不会促进肿瘤的血源性转移,MAGE-1 mRNA可作为评估肝癌疗效的一个重要指标.

Effect of transcatheter hepatic arterial chemoembolization on melanoma antigen-1 mRNA in peripheral blood of patients with hepatocellular carcinoma

Objective To evaluate the effect of transcatheter hepatic arterial chemoembolization (TACE) on melanoma antigen-I (MAGE-I) mRNA expression in peripheral blood of patients with hepatoceltular carcinoma(HCC). Methods RT-PCR was used to detect MAGE-1 mRNA expression in peripheral blood of 18 patients with HCC before and after TACE. The relationship between the positive rate of MAGE-1 mRNA and pathological characteristics was examined. Results Before TACE treatment, peripheral blood positive MAGE-1 mRNA was detected in 10 patients with HCC, and negative MAGE-1 mRNA in the other 8 patients. After TACE treatment, positive expression of MAGE-1mRNA was detected in peripheral blood of 11 patients, and negative in the other 7 patients. The positive rate of MAGE-1mRNA expression was not significantly different before and after TACE treatment (55.6% vs 61.1%, P >0.05). Before and after TACE,the positive rate was associated with the TNM clinical stages (0% vs 76.9%, 20% vs 76.9%, P <0. 05) and extrahepatic metastasis (85.7% vs 36. 4%, 100% vs 36. 4%, P < 0. 05), and was not associated to the tumor size and serum AFP (P > 0. 05). Conclusions TACE may not lead to hematogenous dissemination in the treatment of HCC patients. The expression of MAGE-1mRNA may be used as a marker for evaluating the efficacy of HCC.