基因重组蛛丝蛋白-聚乙烯醇复合支架材料的制备

目的探讨致孔剂NaCl粒径和比例、变性剂和聚乙烯醇(PVA)等因素对基因重组蛛丝蛋白-PVA复合支架材料形态及性能的影响.方法基因重组蛛丝蛋白溶解于98%甲酸,采用冷冻干燥粒子滤沥法制备重组蛛丝蛋白-PVA复合多孔支架;采用扫描电子显微镜观察支架的形态;采用单纤维强力试验机测试支架机械性能.结果乙醇作变性剂制得的多孔支架力学性能较好,支架的断裂应力、断裂比强度均提高5倍以上,断裂伸长率可达12.21%.以粒径<500μm的NaCl为致孔剂制得的多孔支架力学性能较好.高分子材料PVA能明显改善重组蛛丝蛋白多孔支架的件能.结论重组蛛丝蛋白-PVA复合支架材料有望在组织工程领域得以应用.

Preparation of recombinant spider silk protein-polyvinyl alcohol composite scaffold material

Objective To investigate the effects of many factors such as porogen, denaturant and polyvinyl alcohol (PVA) polymer on the structure and properties of recombinant spider silk protein-PVA composite scaffold material. Methods The silk fibrous protein solution was prepared by dissolving the recombinant spider silk protein in 98% formic acid. Recombinant spider silk protein-PVA composite porous scaffolds were prepared by freeze-drying and particle-leaching method. Scanning electron microscope was used to observe the conformation of the scaffolds. Single strand strength testing machine was used to test the mechanical property of the scaffolds. Results Mechanical property of the scaffolds was better with ethanol as denaturant. Breaking stress and break specific tenacity enhanced by over 5 times. Breaking elongation ratio could reach 12.21%. Using NaCt ( ψ<500 μm) as porogen, or the introduction of PVA could improve the performances of porous scaffold evidently. Conclusion Recombinant spider silk protein-PVA composite porous scaffolds will have a prospective application in tissue engineering.