长程体外反搏对高胆固醇血症猪高级氧化蛋白产物和高敏C反应蛋白的影响

目的探讨长程体外反搏对高胆固醇血症猪血清中高级氧化蛋白产物(AOPP)和高敏C反应蛋白(hs-CRP)的影响.方法 18头雄性乳猪随机分为正常饲养组(n=6)、高脂饲养组(n=6)及高脂饲养+体外反搏组(n=6).后2组通过高脂饲养复制高胆固醇血症猪模型并对高脂饲养+体外反搏组进行36 d共36 h的长程增强型体外反搏.分别于分组饲养前、反搏前、反搏中期和反搏结束时留取3组动物静脉血,采用分光光度法检测血清AOPP浓度,采用乳胶凝集反应法检测血清hs-CRP浓度.结果高脂饲养组和高脂饲养+体外反搏组经高脂饲养后血清总胆固醇和低密度脂蛋白明显升高(P<0.05).血清AOPP和hs-CRP浓度在分组饲养前组差异无统计学意义(P>0.05).反搏前、反搏中期和反搏结束时,高脂饲养组与高脂饲养+体外反搏组血清AOPP和hs-CRP浓度较正常饲养组同时期均有显著增高(P<0.05);而反搏中期和反搏结束时高脂饲养+体外反搏组血清AOPP浓度较高脂饲养组显著降低[(95.38±12.66)μmol/L比(128.46±12.55)μmol/L;(85.78±10.33)μmol/L比(158.22±16.32)μmoL/L,P<0.05];且反搏中期和反搏结束时高脂饲养+体外反搏组血清hs-CRP浓度较高脂饲养组也有显著降低[(0.47±0.14)mg/L比(0.62±0.32)mg/L;(0.47±0.16)mg/L比(0.59±0.43)mg/L,P<0.05].结论 AOPP和hs-CRP参与了高胆固醇血症猪的发病过程.长程体外反搏可能通过减轻机体体内氧化应激和微炎性反应过程,从而阻止高胆固醇血症的病理生理进程.

Effect of long-term enhanced external counterpulsation on serum concentrations of advanced oxidation protein products and high-sensitivity C-reactive protein in hypercholesterolemic pigs

Objective To explore the effect of long-term enhanced external counterpulsation (EECP) on serum concentrations of advanced oxidation protein products (AOPP) and high-sensitivity C-reactive protein (hs-CRP) in hypercholesterolemic pigs. Methods Eighteen male infant pigs were randomly divided into 3 groups according to their diet:the normal control group (n=6), the hypercholesterolemic group (n=6) and the hypercholesterolemie + EECP group (n=6). While porcine hypercholesterolemic model was built, EECP was performed on animals of the hypereholesterolemie + EECP group, 2 hours every other day lasting for 36 days. Blood samples were harvested from all animals to detect the levels of AOPP and hs-CRP before initiating different diet, prior to EECP, during EECP and after completion of EECP respectively. Results The serum concentrations of AOPP and hs-CRP, as well as the levels of serum cholesterone and low density lipoprotein, of both the hypereholesterolemic group and the hypercholesterolemic + EECP group were significantly higher than those of normal control group at these time points: prior to EECP, during EECP and after completion of EECP ( P<0.05 ) ,while no difference was observed among three groups before initiating different diet (P>0.05). However, the AOPP levels of the hypereholesterolemic + EECP group were significantly lower compared with those of hypercholesterolemic group during EECP and after completion of EECP respectively[(95.38±12.66 ) μmol/L vs (128.46±12. 55) μmol/L, (85.78±10.33 )μmol/L vs (158.22±16.32) μmol/L, P<0.05], Samely, the hs-CRP concentrations of the hypercholesterolemic + EECP group were also obviously lower compared with those of hypereholesterolemic group during EECP and after completion of EECP respectively[ (0.47±0.14) mg/L vs (0.62±0.32 ) mg/L, (0.47±0.16) mg/L vs (0.59±0.43 ) mg/L, P<0.05]. Conclusions AOPP and hs-CRP play an important role in the pathogenesis of hypercholesterolemia and atherosclerosis. Long-term EECP eliminates the impact of both inflammation and oxidative stress damage from hypercholesterolemia, contributing to postponing initiation and progression of the early atherosclerosis.