IL-12与IL-18在胃癌组织中的表达及其临床意义

目的：探讨胃癌组织中白介素-12（IL-12）与白介素-18（IL-18）的表达，及其与胃癌临床病理特征、血管生成及预后的关系。方法：选取50例胃癌标本，通过免疫组化检测IL-12与IL-18在胃癌组织中的表达，显微图像分析系统测定微血管密度。结果：IL-12与IL-18在胃癌中的阳性表达率分别为44％（22／50）和26％（13／50），IL-12与病理分化（P=0．029）、浸润深度（P=0．002）、淋巴结转移（P=0．002）、远处转移（P=0．003）和TNM分期（P=0．005）相关，IL-18与远处转移密切相关（P=0．001）；多因素生存分析显示IL-12、MVD和T分期是胃癌的独立预后因素。结论：IL-12和IL-18阳性表达与肿瘤进展和转移有关，IL-12是提示胃癌患者预后不良的独立因素。

Expression of interleukin-12 and interleukin-18 in gastric cancer and their clinical significance

Objective:To examine the expressions of interleukin-12 (IL-12) and interleukin-18 (IL-18) in gastric cancer and their association with clinicopathologic features, angiogenesis, and prognosis of gastric cancer. Methods:The expressions of IL-12 and IL-18 in 50 samples of gastric cancer tissues were analyzed by immunohistochemistry. The microvessel density (MVD) was determined with microscopic imaging analysis system. Results:The positive rates of IL-12 and IL-18 expression were 44% (22/50) and 26% (13/50), respectively. IL-12 expression was significantly correlated with pathologic differentiation (P=0.029), depth of invasion (P=0.002), lymph node metastasis (P=0.002), distant metastasis (P=0.003), and TNM stages (P= 0.005). IL-18 expression was closely related to distant metastasis (P=0.001). The multivariate survival analysis revealed that IL-12, MVD and T stage were independent prognostic factors of gastric cancer. Conclusion: The positive expressions of IL-12 and IL-18 correlated with development and metastasis of gastric cancer. IL-12 is an independent factor of poor prognosis of gastric cancer.