Adaptation of a commercially available indirect fluorescent antibody slide test for measuring measles-specific immunoglobulins |
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| Authors: | M B Kleiman C A Bockhold S E Zimmerman C Griffin M L French C Barrett |
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| Affiliation: | 1. Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC), RWTH University Hospital Aachen, D-52074 Aachen, Germany;2. Laboratory Diagnostic Center, RWTH University Hospital Aachen, D-52074 Aachen, Germany;1. Department of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;2. Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan;3. Department of Internal Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan;4. Department of Laboratory Medicine and Pathology, The University of British Columbia, Canada;5. School of Nutrition and Health Sciences, College of Nutrition, Taipei Medical University, Taipei, Taiwan;6. School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan;7. Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan;8. Master Program for Clinical Pharmacogenomics and Pharmacoproteomics, School of Pharmacy, Taipei Medical University, Taipei, Taiwan;9. International Ph.D. Program for Cell Therapy and Regeneration Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan;10. Pulmonary Research Center, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan;1. College of Construction Engineering, Jilin University, Changchun 130026, China;2. Los Alamos National Laboratory, Los Alamos, NM 87545, USA;3. Institute of Nuclear and New Technology, Tsinghua University, Beijing 100084, China;4. Yantai Customs Technology Center, Yantai 264000, China;5. Laboratory for Computational Mechanics, Institute for Computational Science and Artificial Intelligence, Van Lang University, Ho Chi Minh City, Viet Nam;6. MEU Research Unit, Middle East University, Amman, Jordan |
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| Abstract: | A commercially available indirect fluorescent antibody (IFA) test was evaluated for the determination of measles-specific immunoglobulins G and M (MIgG, MIgM). The IFA test detected fourfold rises of MIgG in 34 of 35 (97%) cases of measles confirmed by complement fixation or hemagglutination inhibition. In determining immune status, MIgG-IFA correlated with hemagglutination inhibition in 22 of 23 (96%) cases. The IFA test detected MIgM in only 11 of 34 acute-phase sera collected within 5 days of the reported onset of rash and in the convalescent specimens of another 13 of the 35 specimens. The IFA test is an effective method for the conventional diagnosis of measles and for determining immune status. This IFA test has a limited role as a rapid diagnostic test for measles when used to detect measles-specific MIgM in acute-phase sera obtained from patients with suspected measles. |
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