2011—2013年北京某城区感染性腹泻患者致病菌检测分析

目的分析北京某城区2011—2013年感染性腹泻患者致病菌分布及脉冲场凝胶电泳(PFGE)图谱特征,为感染溯源提供依据。方法收集2011年1月—2013年12月该区域肠道门诊感染性腹泻患者粪便标本1 179份,对其培养分离的致病菌进行血清分型及PFGE分析。结果 1 179份标本,共培养肠道病原菌330株,其中检出居前4位的肠道致病菌依次为志贺菌属93株(28.18%)、沙门菌属69株(20.91%)、副溶血性弧菌44株(13.33%)及致泻性大肠埃希菌11株(3.33%)。18株宋内志贺菌PFGE分为8个型别,聚类相似度接近88%;69株沙门菌属细菌分为18个血清型,共41种PFGE型别,其中山夫登堡沙门菌和肠炎沙门菌具有明显的优势带型;23株副溶血性弧菌PFGE型别较为分散。结论 3年间感染性腹泻患者致病菌的血清型和PFGE带型分布较为广泛,应注意沙门菌属和志贺菌属细菌的PFGE优势菌型,警惕其引发的感染性腹泻暴发流行。

Pathogenic detection of infectious diarrhea in patients in a district of Beijing from 2011 to 2013

ObjectiveTo analyze the distribution and pulsed field gel electrophoresis (PFGE) of pathogenic bacteria causing infectious diarrhea in a district of Beijing from 2011 to 2013, and provide basis for tracing infection sources.MethodsA total of 1 179 stool specimens of infectious diarrhea from patients in a diarrhea outpatient department from January 2011 to December 2013 were collected, all isolated pathogens were identified by serotyping and PFGE analysis.Results330 enteric pathogens were isolated from 1 179 specimens, the top 4 bacteria were Shigella spp.（28.18%,n=93）,Salmonella spp.（20.91%, n=69）,Vibrio parahaemolyticus（13.33%, n=44）,and diarrheagenic Escherichia coli(3.33%, n=11). 18 Shigella sonnei isolates were identified as 8 PFGE patterns, clustering similarity was close to 88%; 69 Salmonella spp. strains belonged to 18 serotypes and 41 PFGE patterns, Salmonella senftenberg and Salmonella enteritidis had dominant patterns; no dominant PFGE patterns were obviously identified among 23 strains of Vibrio parahaemolyticus.ConclusionThe serotypes and PFGE patterns of pathogenic bacteria in infectious diarrhea in past three years showed a wide distribution characteristics, the dominant PFGE patterns of Salmonella spp.and Shigella spp. need to be paid more attention, and outbreak of infectious diarrhea caused by Salmonella spp.and Shigella spp. should be alerted.