In vitro metabolism of the new insecticide flupyrazofos by rat liver microsomes |
| |
| Authors: | H. S. LEE S. JEONG K. KIM J. H. KIM S. K. LEE B. H. KANG |
| |
| Affiliation: | College of Pharmacy, Wonkwang University, Shinyongdong, Iksan 570-749, Korea Toxicology Research Center, Korea Research Institute of Chemical Technology, Yoosung, PO Box 107, Taejeon 305-606, Korea |
| |
| Abstract: | 1. The in vitro metabolism of the new insecticide flupyrazofos was studied using rat liver microsomes. Two metabolites were produced and identified as O, O -diethyl O -(1- phenyl-3-trifluoromethyl-5-pyrazoyl) phosphoric acid ester (flupyrazofos oxon) and 1- phenyl-3-trifluoromethyl-5-hydroxypyrazole (PTMHP) based on UV and mass spectral analysis. 2. Cytochrome P450 oxidatively converted flupyrazofos to flupyrazofos oxon, a major metabolite and phenobarbital-induced microsomes increased this desulphuration by 8- fold. 3. Flupyrazofos oxon was converted to PTMHP with a half-life of 47 8?min by chemical hydrolysis and this conversion also proceeded non-enzymatically under our microsomal incubation conditions. |
| |
| Keywords: | |
|
|
