新生儿病房分离金黄色葡萄球菌耐药性及耐消毒剂基因检测

目的了解新生儿病房送检标本中金黄色葡萄球菌的耐药谱及耐消毒剂基因携带情况。方法收集2013年1—12月某院新生儿病房住院新生儿送检标本分离的金黄色葡萄球菌,采用VITEK2 Compact细菌鉴定分析系统进行细菌鉴定及药敏试验,采用聚合酶链反应(PCR)检测耐消毒剂qacA/B基因。结果新生儿科送检标本中共分离225株金黄色葡萄球菌,主要来源于呼吸道(72.44%)和脐部分泌物(14.33%);耐甲氧西林金黄色葡萄球菌(MRSA)检出率为8.89%(20株);耐药监测中,MRSA的耐药率普遍高于甲氧西林敏感金黄色葡萄球菌(MSSA)。左氧氟沙星、莫西沙星、替加环素、万古霉素、利奈唑胺及呋喃妥因对所有金黄色葡萄球菌均显示出良好的抗菌活性,敏感率均为100%。携带qacA/B基因的金黄色葡萄球菌有21株,阳性率为9.33%,其中MRSA3株(15.00%),MSSA18株(8.78%)。结论新生儿病房分离的MRSA耐药严重,同时携带qacA/B基因,应引起临床重视。

Drug resistance and disinfectant resistant genes of Staphylococcus aureus from neonates at a neonatal ward

ObjectiveTo realize drug resistance pattern and disinfectant resistant genes (qacA/B) of Staphylococcus aureus (S. aureus) from neonates at a neonatal ward.MethodsS. aureus from neonates between January and December 2013 were identified and performed antimicrobial susceptibility testing by VITEK 2 Compact system, qacA/B gene was detected by polymerase chain reaction (PCR). Results225 S. aureus strains were isolated,which were mainly from respiratory tract (72.44%) and umbilical secretion (14.33%); isolation rate of methicillin resistant S.aureus (MRSA) was 8.89%(n=20); antimicrobial resistant rate of MRSA was generally higher than methicillin sensitive S.aureus (MSSA)；levofloxacin, moxifloxacin, tigecycline, vancomycin, linezolid， and nitrofurantoin were all had high antimicrobial activity against all S.aureus， susceptibility rates were all 100%. 21( 9.33%) S.aureus carried qacA/B, 3(15.00%) of which were MRSA, and 18(8.78%) were MSSA.ConclusionDrug resistance of MRSA isolated from neonates in neonatal ward is serious, MRSA strains carry qacA/B, which should be paid more attention.