Molecular modelling of CYP2A enzymes: application to metabolism of the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) |
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| Authors: | J. R. Jalas S. S. Hecht S. E. Murphy |
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| Affiliation: | 1. Department of Chemistry, University of Minnesota, 207 Pleasant Street, SE, Minneapolis, MN, 55455, USA;2. Cancer Center, University of Minnesota, MMC 806, 420 Delaware Street, SE, Minneapolis, MN, 55455, USAmurph062@umn.edu;4. Cancer Center, University of Minnesota, MMC 806, 420 Delaware Street, SE, Minneapolis, MN, 55455, USA;5. Department of Biochemistry, Molecular Biology, and Biophysics, 321 Church Street, SE, Minneapolis, MN 55455, USA |
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| Abstract: | 1.?Tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a lung carcinogen in a variety of animal models and a putative human lung carcinogen. Its tumorigenic potential is unmasked via cytochrome P450 (CYP)-mediated hydroxylation of the carbon atoms adjacent to the nitroso moiety (i.e.?α-hydroxylation). Therefore, elucidation of enzyme–substrate interactions that facilitate?α-hydroxylation is important to gain insight into the tumorigenic mechanism of NNK and to develop potent inhibitors of this detrimental reaction.2.?Molecular models of CYP2A enzymes from mice, rats and humans that are catalysts of NNK bioactivation were constructed and used, in conjunction with docking experiments, to identify active-site residues that make important substrate contacts.3.?Docking studies revealed that hydrophobic residues at positions 117, 209, 365 and 481, among others, play critical roles in orienting NNK in the active site to effect?α-hydroxylation. These molecular models were then used to rationalize the stereo- and regioselectivity, as well as the efficiency, of CYP2A-mediated NNK metabolism. |
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| Keywords: | 2,2′,4,4′,5-Pentabromodiphenyl ether (BDE99) polybrominated diphenyl ether (PBDE) glutathione conjugation glucuronide conjugation sulfate conjugation |
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