HPLC测定复方血栓通胶囊中人参皂苷Rg_1Rb_1和三七皂苷R_1的含量

目的:建立HPLC测定复方血栓通胶囊中人参皂苷Rg1、Rb1和三七皂苷R1含量的方法。方法:采用Shi-madzu-C18柱,以乙腈为流动相A,以水为流动相B,进行梯度洗脱,检测波长为203nm,流速为1.0mL/min,柱温为40℃,理论板数按三七皂苷R1峰计算应不低于2000。结果:测得人参皂苷Rg1在0.868～8.680μg(r=0.9999,n=6)、人参皂苷Rb1在0.892～8.920μg(r=1.0000,n=6)、三七皂苷R1在0.308～3.080μg(r=1.0000,n=6)线性关系良好;平均回收率人参皂苷Rg1为100.11%,RSD0.71%;人参皂苷Rg1为99.91%,RSD0.87%;三七皂苷R1为99.90%,RSD1.61%,(n=5)。结论:本法准确,专属性强。

Determination of Ginsenoside Rg1,Ginsenoside Rb1 and Notoginsenoside R1 in Fufang Xueshuantong Capsules by HPLC

Objective：To establish a HPLC method for determination of the content of ginsenoside Rg1,ginsenoside Rb1 and notoginsenoside R1 in Fufang xueshuantong Capsules.Methods：The contents of ginsenoside Rb1,ginsenoside Rg1 and notoginsenoside R1 were separated by gradient elution on Shimadzu-C18 column （5μm,4. 6mm×100mm）as stationary phase; acetonitrile and water as gradientmixed mobile phase; detected at 203 nm wavelength;40℃ of column temperature and 1. 0mL/min of mobile rate.Results：Ginsenoside Rb1、ginsenoside Rg1 and notoginsenoside R1 all had good linearities at the range of 0.868～8.680μg（r=0.9999,n=6）,0.892～8.920μg（r=1.0000,n=6）and 0.308～3.080μg（r=1.0000,n=6）.The Average recovery rates of ginsenoside Rb1、ginsenoside Rg1 and notoginsenoside were 100.11%,RSD 0.71%;99.91%,RSD 0.87%;99.90%,RSD 1.61%,（n=5）.Conclusion：The method is accurate and specific.