复方中药对人胃癌细胞SGC7901生长抑制作用的研究 |
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引用本文: | 赵永峰,;邹晓明,;侯培珍. 复方中药对人胃癌细胞SGC7901生长抑制作用的研究[J]. 疾病监测与控制, 2008, 0(7): 393-394 |
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作者姓名: | 赵永峰, 邹晓明, 侯培珍 |
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作者单位: | [1]内蒙古医学院第三附属医院,内蒙古包头014010; [2]内蒙古科技大学包头医学院第一附属医院,内蒙古包头014010 |
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摘 要: | 耳的研究复方中药对人胃癌细胞SGC7901生长的抑制作用。方法取对数生长期的人胃癌细胞SGC7901接种96孔板,待细胞生长良好后加入含不同浓度复方中药培养液,取2个时间点(作用24h、48h)采用MTT比色法^[1]观察复方中药对人胃癌细胞SGC7901生长的抑制作用。结果10^4ug/L和10^5ug/L浓度组在24h、48h、均表现出抑制SGC7901细胞生长作用,且作用强于5-Fu。10^3ug/L组在2个时间点也表现出抑制SGC7901细胞生长作用,但作用弱于5-Fu。结论复方中药对人胃癌SGC7901细胞生长有显著的抑制作用,且呈剂量和时间依赖方式。
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关 键 词: | 复方中药 人胃癌SGC7901细胞 抑制作用 |
The effect of CCM to gastric cancer SGC7901 cells |
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Affiliation: | ZHAO Yong-feng, ZHOU Xiao-ming, HOU Pei-zhen(1.The Third Affiliated Hospital of Inner Mongolia Medical College,BaoTou 014010, China; 2.The First Affiliated Hospital of Inner Mongolia Tecbnologe University BaoTou Medical colloge. BaoTou 014010, China) |
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Abstract: | Objective To research the CCM depressant effect about human gastric cancer SGC7901 cells. Methods The SGC7901 cells in logarithm growth phase incubated into 96-well plates.To add the different density CCM culture fluid after cells had better grown.The effect of CCM on SGC7901 cells were observed by MTT assay at Two time points ( 24h.48h ) after culturing medium. Results The inhibition Effects to SGC7901 cells were observed at 24h.48h,in 10^4ug/L and 10^5 ug/L concentration group with higher IRs than 5-Fu control group. The inhibition effects to SGC7901 cells were observed at 24h.48h in 10^3 ug/L concentration group with lower IR than 5-Fu control group. Conclusions The gastric cancer SGC7901 cells were inhibited conspicuous bv the CCM with adose.time-denent manner. |
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Keywords: | CCMG astric cancer SGC7901 cells Depressant effect |
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