| 孔雀绿比色法同步测定大鼠成骨细胞膜Ca^2＋—ATP酶和Na^＋, … |
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| 引用本文： | 岑小波,黄永光.孔雀绿比色法同步测定大鼠成骨细胞膜Ca^2＋—ATP酶和Na^＋, …[J].华西医科大学学报,1998,29(4):427-430. |
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| 作者姓名： | 岑小波 黄永光 |
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| 摘 要： | 为探索同步测定大成骨细胞膜Ｃａ＾２＋－ＡＴＰ酶和Ｎａ＾＋、Ｋ＾＋－ＡＴＰ酶活性的方法。采用孔雀绿比色法测定磷以反映酶活性。结果显示：Ｃａ＾２＋浓度以及成骨细胞传代增减的代数是影响酶活性的重要因素。
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| 关 键 词： | ATPSGTU 成骨细胞 钙 钠 钾 孔雀绿比色法 |
Simultaneous assay of Ca(2+)-ATPase and Na+, K(+)-ATPase activities of osteoblast rat by malachite green colorimetic method] |
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| X Cen,Y Huang,R Wang,Z Chen,Z Wu.Simultaneous assay of Ca(2+)-ATPase and Na+, K(+)-ATPase activities of osteoblast rat by malachite green colorimetic method][J].Journal of West China University of Medical Sciences,1998,29(4):427-430. |
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| Authors: | X Cen Y Huang R Wang Z Chen Z Wu |
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| Institution: | Department of Nutrition and Food Hygiene, School of Public Health, Chengdu. |
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| Abstract: | The aim of this study was to find a method to measure simultaneously the activities of Ca(2+)-ATPase and Na+, K(+)-ATPase of rat osteoblast membrane. In this test, Ca(2+)-ATPase and Na+, K(+)-ATPase released inorganic phosphate (Pi) from ATP, and Pi was determined by colorimetric method using malachite green dye to show ATPase activities. Ca2+ concentration and osteoblast culture generation were important factors affecting ATPase activities. Ca2+ could elevate membrane ATPase activity. When Ca2+ concentration reached 40 mumol/L, ATPase activities were improved. Ca(2+)-ATPase activity decreased with the increase of osteoblast culture generation. Ca(2+)-ATPase activity was lower than Na+, K(+)-ATPase activity in osteoblast membrane. This method is simple, sensitive and reliable. |
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