病理性瘢痕组织iNOS和5-HT表达及与其增生和痛痒的关系

目的 检测诱导型一氧化氮合酶（iNOS）、5-羟色胺（5-HT）在病理性瘢痕及正常皮肤组织中的表达，探讨其与病理性瘢痕增生及痛痒的关系。方法 应用免疫组化技术对痛痒、非痛痒的病理性瘢痕和正常皮肤组织中iNOs、5-HT的表达及分布情况进行检测，并采用图像分析法对检测结果进行半定量分析。结果 痛痒的瘢痕组织与正常皮肤组织相比．iNOS、5-HT的表达均显著升高（F=25．47、44．92，q=4．39、4．43，P〈0．01）；非痛痒的瘢痕组织与正常皮肤组织相比．iNOS表达显著升高（q=3．96，P〈0．01），5-HT表达差异无显著性（q=2．64，P〉0．05）；痛痒的瘢痕组织与非痛痒的瘢痕组织相比，5-HT的表达显著升高（q=3．92，P〈0．01），iNOS的表达差异无显著性（q=2．71，P〉0．05）。结论 iNOS可能与病理性瘢痕的形成有关，其增高可能促进瘢痕的增生；5-HT可能与病理性瘢痕的临床痛痒症状有关，其增高可能导致病理性瘢痕临床痛痒症状的发生。

EXPRESSIONS OF iNOS AND 5-HT IN ABNORMAL SCAR TISSUE AND THEIR RELATIONSHIP WITH ITCHING AND PAIN

ObjectiveTo study the expressions of inducible nitric oxide synthase(iNOS) and 5-hydroxytryptamine(5-HT) in abnormal scar and normal skin and their relationship with the scar proliferation and itching or pain.MethodsImmunohistochemical method was adopted to detect iNOS and 5-HT in itching or painful scars and non-itching or non-painful scars and normal skin tissues,the results were semi-quantitatively analyzed by image analysis.ResultsCompared with normal skin,iNOS and 5-HT expression in itching or painful scars were significantly higher(F=25.47,44.92;q=4.39,4.43;P<0.01),and iNOS in non-itching or non-painful scars was significantly higher(q=3.96,P<0.01),5-HT was no difference(q=2.64,P>0.05).In the itching or painful scars,5-HT was significantly higher than in non-itching or non-painful scars(q=3.92,P<0.01);no difference was noted in iNOS(q=2.71, P>0.05).ConclusioniNOS may correlate with the scarring,and higher expression of iNOS may contribute to it.5-HT may correlate with the itching and painful symptoms of abnormal scars,and higher expressions of it may induce the symptoms.