辅酶Q10对体外培养的小鼠脑微血管内皮细胞凋亡和增殖的影响

目的:研究辅酶Q₁₀抑制血管内皮细胞凋亡和增殖的作用及其可能机制。方法: 用血清药理学方法和流式细胞仪法检测体外培养的小鼠脑微血管内皮细胞(bEnd.3)中不同浓度CoQ₁₀对细胞凋亡和增殖的变化。用免疫细胞化学ABC法观察Fas蛋白及Bcl-2蛋白表达的变化。结果: 与对照组比(加不含药血清),在bEnd.3细胞培养中加入50 μL、25 μL、12.5 μL含CoQ₁₀的血清组细胞凋亡明显抑制(P<0.05)。免疫细胞化学检验结果证明,此时Fas蛋白表达明显减弱,而Bcl-2蛋白表达明显增强(P<0.05)。 但CoQ₁₀对细胞增殖影响不显著。 结论: 一定浓度的CoQ₁₀能通过上调Bcl-2蛋白和下调Fas蛋白表达抑制小鼠微血管内皮细胞凋亡,从而对微血管内皮细胞起保护作用。

Effect of CoQ10 on apoptosis and proliferation of cultured mouse microvascular endothelial cells and it''s mechanism

AIM: To study the inhibitory effect of CoQ₁₀ on the apoptosis of microvascular endothelial cells and it's probable mechanism. METHODS: Using serum pharmacology method and cytoflowmetery, the effects of CoQ₁₀ at different concentrations on apoptosis and proliferation in cultured mouse brain microvascular endothelial cells (bEnd.3) were investigated. The expression of Fas protein and Bcl-2 protein were observed with immunocytochemical method (ABC). RESULTS: The cell apoptosis was inhibited significantly in CoQ₁₀ groups (50 μL and 25 μL) in cultured bEnd.3 cells. The results of immunocytochemical staining showed that the expressions of Fas protein was inhibited and Bcl-2 protein was stimulated significantly in CoQ10 group with above concentration. But there was no significant change in cell proliferation. CONCLUSIONS: CoQ₁₀ may inhibit apoptosis of microvascular endothelial cells (bEnd.3) via up-regulation of Bcl-2 and down-regulation of Fas. Authors suggest that this is one of the protection mechanisms of CoQ₁₀ from dysfunction of microvascular endothelial cells.