雷公藤内酯醇调节5-脂氧合酶对胰腺癌细胞增殖的影响

目的:探讨雷公藤内酯醇(Triptolide,TL)在胰腺癌细胞中5-脂氧合酶(5-LOX)蛋白的表达及对癌细胞生长的影响.方法:应用免疫细胞化学法检测胰腺癌细胞株SW1990和Capan2中5-LOX蛋白的表达,同时检测TL对胰腺癌细胞株SW1990 5-LOX蛋白表达的影响,采用MTT法和流式细胞术分别检测胰腺癌细胞SW1990的增殖和凋亡.结果:胰腺癌细胞株SW1990和Capan2中均表达5-LOX,并与细胞分化程度有关;TL抑制SW1990中5-LOX蛋白的表达,并抑制胰腺癌细胞SW1990增殖和促进细胞的凋亡,作用随浓度的增加而增强(P＜0.01);细胞的增殖和凋亡与5-LOX蛋白的表达相关(P＜0.01).结论:TL抑制胰腺癌细胞SW1990中5-LOX的表达,并抑制胰腺癌细胞增殖和诱导凋亡,5-LOX代谢途径在胰腺癌细胞的生长中发挥重要作用.

The Effect of Triptolide on Cell Proliferation of Pancreatic Cancer Cell by Regulating the Expression of 5-lipoxygenase

Objective: To investigate the effect of Triptolide on the growth of human pancreatic cancer cell by regulating the protein expression of 5-lipoxygenase(5-LOX). Methods: Pancreatic cancer cell lines SW1990 and Capan2 were used in this study. SP immunocytochemical staining was used to detect the protein expression of 5-LOX in the pancreatic cells SW1990 and Capan2. The effect of Triptolide(TL) on pancreatic cancer cell line SW1990 was determined by SP immunocyto-chemical staining, MTT and flow cytometry (FCM). Results: The protein expression of 5-LOX in pancreatic cancer cell lines SW1990 and Capan2 were demonstrated by immunocytochemical staining. Staining for the 5-LOX protein was more evident in the well differentiated pancreatic cancer cell line Capan2 than the moderately differentiated pancreatic cell line SW1990. This study also demonstrated that the protein expression of 5-LOX was inhibited by TL. The cell proliferation was blocked by TL, which showed a significant positive association with the level of TL concentration (P<0.01). The FCM analysis showed that the pro-apoptotic effect of TL on cell line SW1990 was in a concentration-dependent manner. There was correlation between the expression of 5-LOX and proliferation or apoptosis index in our study(P<0.01). Conclusion: TL can inhibit the proliferation and induce apoptosis of pancreatic cancer cells by regulating the expression of 5-LOX protein.