褪黑素对病毒唑体外抗呼吸道合胞病毒的协同作用研究

目的:研究褪黑素对病毒唑体外抗呼吸道合胞病毒(RSV)的协同作用。方法:采用MTT比色法,观察2种药物联用时对RSV在人喉癌上皮细胞(Hep-2)所致细胞病变的抑制效应,统计学及金正均法q值判断2种药物的相互作用。结果:病毒唑对RSV具有抑制作用,且呈浓度依赖的趋势;褪黑素几乎没有抗RSV活性,但能增强病毒唑对RSV的抑制作用;2种药物合用对病毒的抑制作用明显增强,褪黑素(25、50mg·L-1)分别与病毒唑(50、100、200mg·L-1)合用时,q值均>1.15,呈现协同作用。结论:病毒唑与褪黑素联合可协同增强对RSV的抑制作用。

Synergy Effect of Melatonin on Anti - respiratory Syscytical Virus Activity of Ribavirin in Vitro

OBJECTIVE:To study the effect of melatonin in enhancing anti-respiratory syscytical virus (RSV) activity of ribavirin in vitro. METHODS: The anti-RSV effect on Hep-2 cell line of melatonin and ribavirin in combination use was observed by MTT colorimetric assay,and the interaction of melatonin and ribavirin was estimated by q value provided by Jin Zhengjun’s method. RESULTS: Ribavirin was effective in inhibiting RSV, and the effect was improved with its concentration increasing; melatonin has little activity on RSV resistance,but it can enhance the anti-RSV effect of ribavirin. The inhibition of MT and ribavirin on RSV was significantly enhanced,and the synergistic effect was observed when melatonin (25, 50mg·L-1) was used in combination with ribavirin (50,100, 200mg·L-1), with q values>1.15. CONCLUSIONS: Ribavirin is effective in inhibiting RSV in vitro and the effect is better when used in combination with melatonin.