应用数据挖掘技术筛选的抗癌中药对三阴性乳腺癌细胞增殖、迁移的影响

【目的】基于数据挖掘技术筛选具有较明显抑制三阴性乳腺癌作用的中药。【方法】收集近15年关于乳腺癌中医治疗的有效文献,采用频次和聚类分析确定高频中药,制备其含药血清,采用四甲基偶氮唑盐(MTT)法和Transwell小室侵袭、迁移实验,分别观察各高频中药含药血清作用三阴性乳腺癌细胞(MDB-MB-231)12、24 h后细胞增殖、迁移和侵袭能力变化。【结果】筛选出的高频使用中药为川芎、蒲公英、白花蛇舌草、天花粉、牛膝。这5种中药的含药血清对MDB-MB-231细胞的增殖能力均具有不同程度的抑制作用,其中蒲公英、白花蛇舌草、牛膝对MDB-MB-231细胞迁移能力具有较强的抑制作用,蒲公英、川芎、白花蛇舌草对MDB-MB-231细胞侵袭能力具有明显抑制作用。【结论】运用数据挖掘技术得到的高频中药川芎、蒲公英、白花蛇舌草、天花粉、牛膝等具有明显的抑制三阴性乳腺癌作用。

Effects of Anticancer Chinese Medicines Screened Out by Data Mining on Proliferation and Migration of Triple-negative Breast Cancer Cells

Objective To screen out some Chinese medicines with obvious inhibitory actions on triple-negative breast cancer based on data mining. Methods Literatures of breast cancer effectively treated by Chinese medicine issued in recent 15 years were collected, and then the frequency analysis and cluster analysis were used to screen the frequently-used herbs. According to the above results, the serum containing the obtained drugs were prepared respectively. And then the proliferation, migration and invasion abilities of breast cancer cells MDB-MB-231 treated with the serum containing various Chinese medicines for 12, 24 h were observed by methyl thiazolyl tetrazolium(MTT) assay and transwell cell invasion and migration experiments, respectively. Results The frequently-used herbs were screened out as Rhizoma Chuanxiong, Herba Taraxaci, Herba Hedyotis Diffusae, Radix Trichosanthis, Radix Achyranthis Bidentatae. The proliferation of MDB-MB-231 cells was significantly inhibited by serum seperately containing Rhizoma Chuanxiong, Herba Taraxaci, Herba Hedyotis Diffusae, Radix Trichosanthis, and Radix Achyranthis Bidentatae to different degrees, and the migration abilities of MDB-MB-231 cells were obviously inhibited by serum containing Herba Taraxaci, Herba Hedyotis Diffusae, Radix Achyranthis Bidentatae, respectively. And the invasion abilities of MDB-MB-231 cells were obviously inhibited by serum containing Herba Taraxaci, Rhizoma Chuanxiong, Herba Hedyotis Diffusae, respectively. Conclusion Chinese medicines such as Rhizoma Chuanxiong, Herba Taraxaci, Herba Hedyotis Diffusae, Radix richosanthis, Radix Achyranthis Bidentatae obtained by data mining technology have obviously inhibitory effects on triple-negative breast cancer.