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| Schlafen2基因在细胞中的表达及其生物学特性研究 | |
| 引用本文： | 刘梦伊,周乔丹,胡迎春,方玲,于雷,张煦,刘昕,潘兴斌,吴德昌,霍艳英.Schlafen2基因在细胞中的表达及其生物学特性研究[J].军事医学科学院院刊,2008,32(3). |
| 作者姓名： | 刘梦伊 周乔丹 胡迎春 方玲 于雷 张煦 刘昕 潘兴斌 吴德昌 霍艳英 |
| 作者单位： | 1. 兰州大学基础医学院病理教研室,兰州,730000;军事医学科学院放射与辐射医学研究所,北京,100850 2. 重庆医科大学病理学教研室,重庆,400016 3. 军事医学科学院放射与辐射医学研究所,北京,100850 4. 兰州大学基础医学院病理教研室,兰州,730000 |
| 摘 要： | 目的:建立Schlafen2(Slfn2)基因稳定表达的细胞系以进一步研究其生物学功能.方法:构建pEGFP- Slfn2真核表达载体;瞬时转染pEGFP- Slfn2载体及其对照载体于NIH/3T3细胞,观察细胞中Slfn2基因的表达及分布;稳定转染pEGFP-Slfn2及其对照空载体于NIH/3T3细胞,G418筛选获得抵制的细胞株,用Northern印迹进一步鉴定Slfn2在各细胞株中的表达情况;利用细胞生长曲线和Transwell细胞侵袭实验检测Slfn2对细胞增殖及迁移能力的影响.结果:本研究获得了稳定表达Slfn2的细胞株,该基因表达的蛋白在细胞核及细胞浆均有分布;稳定表达该基因的细胞株的增殖及迁移能力均显著降低.结论:Slfn2基因可能是一个潜在的肿瘤抑制基因. |
| 关 键 词： | 细胞增殖 迁移 转染 基因表达 |
Expression of Schlafen2 gene and its effect on NIH/3T3 cells |
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| LIU Meng-Yi,ZHOU Qiao-Dan,HU Ying-Chun,FANG Ling,YU Lei,ZHANG Xu,LIU Xin,PAN Xing-Bin,WU De-Chang,HUO Yan-Ying.Expression of Schlafen2 gene and its effect on NIH/3T3 cells[J].Bulletin of the Academy of Military Medical Sciences,2008,32(3). | |
| Authors: | LIU Meng-Yi ZHOU Qiao-Dan HU Ying-Chun FANG Ling YU Lei ZHANG Xu LIU Xin PAN Xing-Bin WU De-Chang HUO Yan-Ying |
| Institution: | LIU Meng-Yi1,2,ZHOU Qiao-Dan3,HU Ying-Chun2,FANG Ling1,YU Lei2,ZHANG Xu1,LIU Xin1,PAN Xing-Bin1,WU De-Chang2,HUO Yan-Ying2(1.Department of Pathology,Preclinical Medical College of Lanzhou University,Lanzhou 730000,China,2.Beijing Institute of Radiation Medicine,Beijing 100850,3.Department of Pathology,Chongqing University of Medical Sciences,Chongqing 400016,China) |
| Abstract: | Objective:To establish cell lines which can stably express Schlafen2(Slfn2)gene and to elucidate biological functions of the gene.Methods:pEGFP-Slfn2 eukaryotic expressing vector was constructed. Control and pEGFP-Slfn2 vectos were transiently transfected into NIH/3T3 cells to observe the distribution of Slfn2 protein directly by laser scanning confocal microscopy. Control and pEGFP-Slfn2 were stably transfected into NIH/3T3 cells and G418 screening was used to obtain resistant cell strains. The expression ... |
| Keywords: | Schlafen2 |
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