实时荧光定量聚合酶链反应检测解脲支原体感染

目的采用实时荧光定量聚合酶链反应(FQ-PCR)技术,准确定量检测待检标本中解脲支原体(UU)的感染状况。方法采用实时FQ-PCR技术,检测标本765份,并用常规PCR技术,对比检测了85例。结果FQ-PCR检测的765份标本,UU-DNA阳性279份,阳性率36.5％,其DNA平均拷贝数为2.4×104。38例阳性患者治疗2周内复查,转阴率为44.7％,治疗3周后复查,转阴率76.0％(P＜0.005)。常规PCR结果重复检测时,25例阳性者中2例变为阴性,而60例阴性者中4例变为阳性。FQ-PCR结果重复时完全吻合。结论实时FQ-PCR检测UU是一个较好的方法。

Fluorescence Quantitative PCR Detected Infection of Ureaplasma Urealyticum

Objective Infection of ureaplasma urealyticum(UU) was detected byreal time FQ-PCR technology.Methods 765 cases specimens of UU-DNA quantification were examined by automated of real time quantification with the ABI Prism 7700 Sequece Detector.Comparison detected 85 cases by FQ-PCR and ordinary PCR.Results UU-DNA was found in 279 of 765(36.5%).DNA average amount of positive samples was 2.4×104.38 cases were examined in treatment two week,negative cases 17 of 38(44.7%),after treatment three week,negative cases 29 of 38(76%),P＜0.005.2 cases changed from positivity to negativity in 25 cases and 4 cases changed from negativity to positivity in 60 cases when Results of ordinary PCR was repeated.Conclusion Real time FQ-PCR is a good method for measuring UU load.