| 沙眼衣原体抑制etoposide诱导的HeLa229细胞凋亡的研究 |
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| 引用本文： | 杨志英,张贤建,欧录明,余平.沙眼衣原体抑制etoposide诱导的HeLa229细胞凋亡的研究[J].中国现代医学杂志,2008,18(16):2336-2339,2343. |
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| 作者姓名： | 杨志英 张贤建 欧录明 余平 |
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| 作者单位： | 1. 湘南学院基础医学课部,湖南,郴州,423000
2. 中南大学免疫学系,湖南,长沙,410078 |
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| 摘 要： | 目的 探讨沙眼衣原体D血清型感染对宿主细胞凋亡的影响.方法 用凋亡诱导剂依托泊苷(e-toposide)作用于沙眼衣原体(D血清型)感染的和未感染的Hela229细胞,Hoechst33258染色后荧光显微镜观察核浓缩和凋亡小体,流式细胞仪检测细胞周期和凋亡率.结果 未感染CT的Hela229细胞经etoposide作用后,用荧光显微镜观察到明显的核浓缩和凋亡小体;流式细胞仪检测的凋亡率为90.64%,与未经诱导剂作用的Hela229细胞比较差异有显著性(P<0.05).而CT感染24 h的Hela229细胞,经etoposide作用后未观察到核浓缩和凋亡小体;流式细胞仪检测的凋亡率为11.50%,与未感染CT的Hela229细胞诱导后的凋亡率比较差异有显著性(P<0.05);而与未经诱导的CT感染Hela229细胞比差异较无显著性(P>0.05).结论 沙眼衣原体感染Hela229细胞后能抑制etoposide诱导的细胞凋亡.
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| 关 键 词： | 沙眼衣原体(D血清型) Hela229细胞 凋亡 |
Study on chlamydia trachomatis inhibition of apoptosis induced by etoposide in Hela229 cell |
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| YANG Zhi-ying,ZHANG Xian-jian,OU Lu-ming,YU Ping.Study on chlamydia trachomatis inhibition of apoptosis induced by etoposide in Hela229 cell[J].China Journal of Modern Medicine,2008,18(16):2336-2339,2343. |
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| Authors: | YANG Zhi-ying ZHANG Xian-jian OU Lu-ming YU Ping |
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| Institution: | YANG Zhi-ying1,ZHANG Xian-jian1,OU Lu-ming1,YU Ping2 (1.Department of Basical Medicine,Xiangnan University,Chenzhou,Hunan 423000,P.R.China,2.Department of Immunology,Central South University,Changsha,Hunan 410078,P.R.China) |
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| Abstract: | Objective] To explore the effect of C. trachomatis (serovar D)infection on apoptosis of host cell. Methods] Induced by etoposide, Detection of cell apoptosis: Nuclear condensation and apoptotic bodies were observed by fluorescence microscope after Hoechst 33258 stain. Apoptotic index were analyzed by flow cytometer assay (FCM). Results] Induced by etoposide, uninfected Hela229 cells showed nuclear condensation and apoptosis bodies. FCM showed that the apoptotic rate was 90.64% in uninfected Hela229 cells... |
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| Keywords: | chlamydia trachomatis (serovar D) Hela229 cell apoptosis |
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