力竭性游泳对大鼠房室结糖原和酶组织化学的影响

目的:探讨力竭性游泳对大鼠房室结(AVN)糖原和酶组织化学的影响。方法:建立大鼠力竭性游泳模型,运用组织化学技术,结合数字图像分析方法,观测房室结糖原(Gly)含量和乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)、单胺氧化酶(MAO)和乙酰胆碱脂酶(ACHE)分布与含量的变化。结果:与对照组相比,1次力竭性游泳后房室结的Gly含量下降,LDH活性增强,SDH、MAO和ACHE的活性均减弱;与1次力竭组相比,1周力竭后Gly含量上升,LDH、SDH和MAO活性均减弱,ACHE活性增强。结论:力竭性游泳对房室结的糖原和酶组织化学的影响较为明显,这可能是造成房室结功能改变的因素之一。

Effects of exhaustive swimming on glycogen and enzyme-histochemistry of atrioventricular node in rat

Objective: To explore the effect of exhaustive swimming on glycogen and enzyme-histochemistry of atrioventricular node of rat. Methods: Models of exhaustive swimming were established in rats. Histochemistry and digital image analysis methods were used to investigate the glycogen (Gly) content and the activity of lactate dehydrogenase (LDH), succinate dehydrogenase (SDH), monoamine oxidase (MAO) and acetylcholinesterase (ACHE). Results: Compared with the normal control group, the glycogen content in AVN of single exhausted swimming group was reduced. The activity of LDH was increased, whereas that of SDH, MAO and ACHE was decreased. Compared with the single exhausted swimming group, the glycogen content in AVN of the seven consecutive daily exhausted swimming group was increased. The activity of LDH, SDH and MAO was decreased, whereas that of ACHE was increased. Conclusion: The effects of exhaustive swimming on glycogen and enzyme histochemistry of rat atrioventricular node were very evident, and the former may be one of the important factors causing heart rhythm disorders.