| 致病隐球菌DNA中鸟嘌呤加胞嘧啶摩尔百分比含量的研究 |
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| 引用本文： | 赵志强,廖万清. 致病隐球菌DNA中鸟嘌呤加胞嘧啶摩尔百分比含量的研究[J]. 中华皮肤科杂志, 1991, 24(5): 323-325 |
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| 作者姓名： | 赵志强 廖万清 |
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| 作者单位： | 中国人民解放军第二军医大学长征医院皮肤科 |
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| 摘 要： | 用热变性法测定了致病隐球菌DNA核普酸组成中的硷基比率.结果罗伦隐球菌最低,新型隐球菌最高,新型隐球菌上海变种介于罗伦隐球菌和新型隐球菌格特变种之间.其变异范围为47.82-61.98.用蜗牛酶辅以十二烷基磺酸钠(SLS)破壁,克服了隐球菌壁厚且有夹膜难以提取DNA的问题.
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| 收稿时间： | 1990-03-12 |
Determination of Guanine and Cytosine Contents of DNA in Some Pathogenic Cryptococci |
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| ZHAO Zhi-Qiang,LIAO Wan-Qing. Determination of Guanine and Cytosine Contents of DNA in Some Pathogenic Cryptococci[J]. Chinese Journal of Dermatology, 1991, 24(5): 323-325 |
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| Authors: | ZHAO Zhi-Qiang LIAO Wan-Qing |
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| Affiliation: | Chang Zheng Hoapital, Shanghai |
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| Abstract: | The G+C mol% content of some species of Cryptococei was reported here as determined by a thermal denaturation iechninue.The results showed that the G+C mol% content was lowest in C.lurentii,highest in C.neoformans,and Shanghai variant between C.laurentii and C.gatlii variant the range of them was from 47.82 to 61.98%,which are similar to those reported in the literatures. |
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