| 高效液相色谱法测定沙苑子及其炮制品中沙苑子苷的含量 |
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| 引用本文： | 贺成,唐晓晶,陈玉武,秦文杰,叶祖光. 高效液相色谱法测定沙苑子及其炮制品中沙苑子苷的含量[J]. 中国中医药信息杂志, 2010, 17(3): 49-51 |
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| 作者姓名： | 贺成 唐晓晶 陈玉武 秦文杰 叶祖光 |
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| 作者单位： | 中药复方新药开发国家工程研究中心,北京,100075 |
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| 摘 要： | 目的建立沙苑子中沙苑子苷的含量测定方法,考察沙苑子炮制前后沙苑子苷的含量变化。方法采用高效液相色谱法,Apollo-C18(4.6mm×250mm,5μm)色谱柱,以乙腈-0.1%磷酸溶液(21∶79)为流动相,流速1.0mL/min,检测波长为266nm。结果沙苑子苷在0.0416～0.6656μg范围内线性关系良好(r=0.9999),平均加样回收率为100.6%,RSD=0.73%。结论本法简便、准确、重复性好、专属性强,为沙苑子药材质量控制提供了方法。盐炙可导致沙苑子中沙苑子苷含量下降。
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| 关 键 词： | 沙苑子 沙苑子苷 高效液相色谱法 含量测定 |
Determination of Complanatuside in Semen Astragali Complanati and Its Processed Products by HPLC |
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| Affiliation: | HE Cheng, TANG Xiao-jing, CHEN Yu-wu, et al (National Engineering Research Center for R﹠D of TCM Multi-ingredient Drugs, Beijing 100075, China) |
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| Abstract: | Objective To establish a method for the determination of complanatuside in Semen Astragali Complanati, and study the content change of complanatuside processed. Method HPLC was used with Apollo-C18 column （4.6 mm×250 mm, 5 μm）, acetonitrile-0.1%phosphoric acid （21∶79） as mobile phase, the flow rate at 1.0 mL/min, and detected wavelength was set at 266 nm. Result Complanatuside showed a good linearity relationship in the range of 0.041 6～0.665 6 μg, r =0.999 9. The average recovery was 100.6%, RSD was 0.73%. Conclusion The method is simple, accurate, reproducibility and strong specificity. It can be used for the quality control of Semen Astragali Complanati. Processing with salt can lead to content decrease of complanatuside in Semen Astragali Complanati. |
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| Keywords: | Semen Astragali Complanati complanatuside HPLC content determination |
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