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| 高效液相法测定加替沙星尿药浓度 | |
| 引用本文： | 邓立东,程强,徐勤.高效液相法测定加替沙星尿药浓度[J].右江民族医学院学报,2002,24(3):344-345. |
| 作者姓名： | 邓立东 程强 徐勤 |
| 作者单位： | 1. 广西桂林医学院附属医院,广西,桂林,541001 2. 四川抗菌素工业研究所,四川,成都,610051 3. 广西桂林医学院药物分析教研室,广西,桂林,541001 |
| 摘 要： | 目的 建立反相高效液相色谱法以测定大鼠尿液中加替沙星浓度。方法 尿液样品用二氯甲烷提取 ,以环丙沙星为内标 ,色谱柱 :LichrosorbC1 8,流动相 :甲醇 - 0 .0 1mol/L ,磷酸二氢钾缓冲液 - 0 .5mol/L ,四丁基氢氧化铵溶液 (1 5∶75∶1 .4) ,磷酸调 pH至 2 .6 ;流速为 1 .0ml/min ;检测波长 2 98nm。 结果 本法在 3 .1 0～ 1 55 .2 μg/ml范围内线性良好 ,r=0 .9995 ,日内RSD为 5 .1 %～ 9.7% ,日间RSD为 5 .7%～ 1 0 .2 % (n =5) ;尿液最低检测浓度为 50ng/ml。 结论 本法测试快速、准确、重现性好 |
| 关 键 词： | 色谱法 高压液相 抗感染药 喹诺酮 大鼠 尿 药代动力学 |
| 文章编号： | 1001-5817(2002)03-0344-02 |
| 修稿时间： | 2002年3月25日 |
High Pressure Liquid Chromatography in detection of Gatifloxacin concentration in rat urine |
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| DENG Li-dong , CHENG Qiang , XU Qin.High Pressure Liquid Chromatography in detection of Gatifloxacin concentration in rat urine[J].Journal of Youjiang Medical College For Nationalities,2002,24(3):344-345. | |
| Authors: | DENG Li-dong CHENG Qiang XU Qin |
| Institution: | DENG Li-dong 1, CHENG Qiang 2, XU Qin 3 |
| Abstract: | Objective To establish a reversed phase High Pressure Liquid Chromatography (HPLC) Method in determination of Gatifloxacin concentration in rat urine. Methods The urine samples were extracted by dichloromethane solvent. Column: Lichrosorb C 18 ; mobile phase: mehthanol-0.01mol/L, potassium dihygrogen phosphate- 0.5mol/L, tetra-butylammiun hydroxygen (15∶75∶1.4), pH was adjusted to 2.6 with phosphoric acid; flow rate: 1.0 ml/min; detection wave length: 298nm. Results Assay linearity was obtained in the range of 3.10～155.2μg/ml, r=0.9995, intra-day and inter-day RSD were 5.1%～9.7% and 5.7%～10.2% (n=5), respectively; the lowest detectable concentration was 50ng/ml. Conclusions This method is rapid, accurate and good in reappearance. |
| Keywords: | chromatography high pressure liquid anti-infective agents quinolone rats urine pharmacokinetics |
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