A new simply and effective fractionation method for cylindrospermopsin analyses. |
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Authors: | Takuya Kubo Tomoharu Sano Ken Hosoya Nobuo Tanaka Kunimitsu Kaya |
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Affiliation: | Graduate School of Environmental Studies, Tohoku University, Aramaki-aza aoba 6-6-20, Aoba-ku, Sendai 980-8579, Japan. kubo@mail.kankyo.tohoku.ac.jp |
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Abstract: | A new simply and effective fractionation method for cylindrospermopsin (CYN) analyses was developed. The extract from cells of Cylindrospermopsis raciborskii was resuspended with 0.1 M carbonate buffer at pH 10.5, and pass through the double-cartridges column which was consisted of a styrene polymer cartridge and an anion exchange cartridge. CYN and deoxy-CYN were adsorbed with the anion exchange cartridge. After separation of the anion exchange cartridge, adsorbed compounds were eluted from the cartridge with 50% methanol containing 1% formic acid solution. CYN and deoxy-CYN were selectively condensed in the eluted solution. When CYN was analyzed by LC-photodiode array or LC/MS, only two peaks of CYN and deoxy-CYN were detected quantitatively. The results suggest that the fractionation method is a useful method for CYN analyses and must be utilized for CYN purification. |
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Keywords: | CYN Fractionation Double-cartridges |
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