Differential activation of mitogen—activated protein kinases by γ—irradiation in IEC—6 cells:Role of intracelluklar Ca^2+ |
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作者姓名: | 周舟 IgishHideki 等 |
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作者单位: | [1]InstituteofCombinedInjury,ThiredMilkitaryMedicalUiversity,Chongqing400038,China [2]DepartmentofEnvironmentalToxicology,UniversityofOccupationalandEnvironmentalHealth,Kitakyushu807-8555,J |
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摘 要: | Abstract Objective:To explore the effects of γ-irradiation on mitogen-activatedprotein kinases(MAPKs) and role intracellular calcium in this event in intestinal epithelial cell line 6(IEC-6 cells).Methods:After cultured rat IIEC-6 cells with or without the pretreatment of intracellular Ca^2 chelator were exposed to γ-ir-radiation of 6 Gy, the total and phosphorylated MAPKs in the cells were determined with Western blotting and apoptosis was examined with flow cytometry.Activities of Extracellular signal-regulated protein kinase (ERK) and p38 MAPK were determined by using immuoprecipitation followed by Western blotting.Results:In response to γ-irradiation,phosphorylation of ERK was not significantly observed ,while the levels of phos-phorylated c-Jun NH2-terminal kinase(JNK) and p38 MAPK were increased in 30 min and reached the peak 2h after exposure to 6Gy γ-irradiation,though the cell viability was significantly lowered 12h.On the other hand, no obvious changes were seen in the total protein levels of ERK, JNK and p38 MAPK.Chelation of in-tracellular Ca^2 almost completely suppressed the JNK and p38 MAPK phosphorylation induced by γ-irradiation is a potent activator for JNK and p38 MAPK, and Ca^2 mobilized from intracellular stores plays an important role in the activation of MAPKs and the induction of apoptosis in IEC-6 cells.
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关 键 词: | 分裂素活化蛋白激酶 活化差异 细胞凋亡 γ-辐射 IEC-6细胞 细胞内钙离子 信号转导 |
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