环磷酰胺对大鼠脑出血血肿周围组织炎症反应的影响

目的研究环磷酰胺对大鼠脑出血血肿周围组织NF-κB表达和白细胞浸润的影响。方法健康雄性SD大鼠125只，随机分成3组：假手术组（25只）、对照组（50只）、治疗组（50只），采用Fredrik建立的自体股动脉取血并向大鼠尾壳核注射方法复制脑出血模型。各组按术后6，24，48，72h及5d分为5个时间点，经尾壳核行冠状切片，分别按干湿重法测脑含水量，免疫组织化学法测定NF—κB的表达，HE染色测定血肿周围组织白细胞浸润。结果假手术组各时间点NF-κB表达及白细胞浸润无明显变化。治疗组除6h、24h外，各时间点血肿周围脑组织含水量、白细胞浸润及NF-κB表达均低于对照组（P〈0．05）。NF-κB表达与白细胞浸润呈现同步性，而且NF-κB的表达高峰时间先于血肿周围组织白细胞浸润。结论环磷酰胺具有减低NF-κB活性．抑制脑出血血肿周围组织的炎症反应起到神经僳护作用．

Influence of Cyclophosphamide on Inflammatory Response of Brain Perihematoma Region in Rats with Cerebral Hemorrhage

Objective To study the influence of cyclophosphamide on expressions of nuclear factor（NF）-κB and Leukocytic infiltration in the brain perihematoma region in rats with cerebral hemorrhage. Methods 125 health male rats were randomly divided into false-operation group （ n = 25 ）, therapy group （ n = 50） and control group （ n = 50 ）. Each group was further divided into 5 subgroups according to the time of 6,24,48,72 h and 5 d after model creation. The intracerebral hemorrhage model was duplicated with the method created by Fredrik. Brain water content was measured. Expressions of NF-κB and Leukocytic infiltration were assayed in each group by immunohistochemical method. Results The difference of NF-κB expression and Leukocytic infiltration was not significant in false operation group. Brain water content was lower in therapy group than that in control group （P 〈 0.05 ） ; In the perihematoma region,positive cells of NF-κB and Leukocytic infiltration in therapy group were more than that in control group（ P 〈0. 05） except 6 h and 24 h. There was synchronism between express of NF-κB and express of Leukocytic infiltration. But the peak of NF-κB expression was earlier than that of Leukocytic infiltration. Conclusion Cyclophosphamide has the effect of brain protection through decreasing expressions of NF-κB and refraining inflammatory reaction.