ITP小鼠模型中NO/NOS对ROS生成影响作用的研究

目的探讨特发性血小板减少性紫癜（ITP）模型小鼠体内,一氧化氮/一氧化氮合酶（NO/NOS）对活性氧（ROS）生成的影响及意义。方法 ITP模型小鼠分成NO供体药物硝普钠（SNP）组和磷酸盐缓冲液（PBS）对照组,SNP组用相同浓度（1mg/ml）的NO供体药硝普钠稀释液进行腹腔注射,PBS对照组使用相同体积的磷酸盐缓冲液进行腹腔注射,用药第5min,15min,25min,35min和45min后,ELISA法检测外周血中NO、NOS的表达及ROS浓度。结果 SNP组小鼠血清中NO及NOS在第5min,15min,25min,35min和45min的浓度均高于PBS对照组（均P〈0.05）。在SNP组中,NO含量及NOS活性随着用药时间的延长而升高,各测量时间点NO含量及NOS活性均具有统计学差异（均P〈0.05）,ROS浓度随着时间的延长而增加（均P〈0.05）,NO表达量与ROS浓度呈正相关（r=0.76,P〈0.05）,NOS表达量与ROS浓度也呈正相关（r=0.82,P〈0.05）。结论在ITP小鼠模型内,NO供体药物硝普钠以时间依赖的形式增加ROS的生成,硝普钠可能通过ROS途径来发挥相应的生理功能。

Study on increased ROS by NO/NOS in ITP mouse model

Objective To investigate the impact and significance of reactive oxygen species （ROS） increased by ni- tric oxide/nitric oxide synthase （NO/NOS） in idiopathic thrombocytopenic purpura （ITP） model mice. Methods ITP model mice were divided into the NO donor drug sodium nitroprusside （SNP） groups, and phosphate buffered saline （PBS） control group,the SNP group with the same concentration （1 mg/ml） of NO donor drug nitroprusside dilution intraperitoneal injection while PBS control group using the same volume of phosphate-buffered saline intraperitoneal in- jection when 5 rain, 15 min, 25 rain, 35 min and 45 min after medication, to detect the expression of NO and NOS in pe- ripheral blood of model ITP mice by ELISA. ROS concentrations were detected by ELISA also at the same time.Results Serum levels of NO and NOS in 5 min,15 min,25 min,35 min and 45 min were higher in SNP group than PBS con trol group （all P〈0.05）. NO content and NOS activity increased with the prolongation of the medication time, NO con- tent and NOS activity was a significant difference among each measurement point （all P〈70.05） in SNP group, ROS concentration increased with time （all P〈~0.05）, NO expression levels and ROS concentration were positively correla- ted （r=0.74, P~0.05）, NOS expression and ROS concentrations also showed a positive correlation （r= 0.81, P〈 0.05）. Conclusion NO donor drug sodium nitroprusside increased generation of ROS in a time dependent form in ITP mouse model, sodium nitroprusside may play a physiological function via ROS pathway.