初诊2型糖尿病患者血清中miR-29a和miR-375表达变化及其与糖、脂标志物相关性研究

目的探讨miR-375和miR-29a在初诊2型糖尿病（DM）患者血清中的表达水平,及其表达变化与糖、脂标志物的相关性。方法排除既往已诊断DM的健康体检者,所有研究对象均行75克葡萄糖耐量（OGTT）实验,根据1999年WHO的DM诊断标准,将研究对象分为糖代谢正常组（38例）和DM组（48例）。以RNU6B作为内参基因,采用实时荧光定量PCR的方法检测血清中miR-29a和miR-375的相对表达水平。结果 DM患者血清中的miR-29a和miR-375表达量皆显著高于对照组。血清中miR-29a和miR-375的表达量与FPG、2hPG和糖化血红蛋白皆呈显著正相关（P〈0.05）,与血脂各指标无显著相关性（P〉0.05）。血清miR-29a和miR-375表达量间呈显著正相关性。结论 miR-29a和miR-375在血清中表达变化有可能作为诊断2型糖尿病的分子标志物。miR-29a和miR-375两者在2型糖尿病的发生、发展中可能具有协同调控作用。

The change of serum miR-375 and miR-29a and their correlation with glycemic control and lipid profile in patients with newly diagnosed type 2 diabetes

Objective To explore the change of serum miR-375 and miR-29a and their correlation with biomarkers of glycemic control and lipid profile in patients with newly diagnosed type 2 diabetes （DM）. Methods The subjects who have been diagnosed DM were screened out in the present study; 48 cases of newly diagnosed DM patients and 38 cases of normal control,after underwent 75g oral glucose tolerance test （OGTT） , were recruited to this study according to the diabetes diagnosis criteria of W/-/O （1999）. Expression of serum miR-29a and miR-375 were measured by real time fluorescent quantitative PCR,normalized to RNU6B. Results Both expression of serum miR-29a and miR-375 were sig- nificantly elevated in DM compared with in normal control. There was a strong correlation between serum miR 29a ex- pression and FPG, 2hPG and haemoglobin Alc, as well as the serum miR-375. No significant relationship of both of ser um miR-375 and miR-29a expression with biomarkers of lipid profile were observed （P〉0. 05）. Moreover,there was a significant correlation of serum expression between serum miR-29a and miR-375. Conclusion Both of serum miR-29a and miR-375 may be useful biomarkers for the screening of type 2 diabetes. The miR-29a and miR-375 may have syner- gistic effect on regulating the pathogenesis of type 2 diabetes.