大鼠慢性高眼压青光眼模型的构建及鉴定

目的:手术建立慢性高眼压青光眼大鼠模型对研究青光眼发病机制、治疗和视神经保护这三方面的研究尤为必要。方法:烧灼3条表浅巩膜静脉,并术中联合应用结膜瓣下浸有0.2g/L的丝裂霉素的凝胶海绵,阻断大鼠的眼部房水外流,建立大鼠慢性高眼压模型,手术后2h;1,3,7,14,28,56d处死5只大鼠,空白对照组亦处死5只大鼠;摘除眼球光镜下进行视网膜形态的观察及视网膜厚度测量,研究眼压、视网膜神经节细胞(retinal ganglion cells,RGC)和视神经损伤的特性。结果:大鼠手术眼与假手术眼、空白对照眼的术后各时间点的眼压比较差异有显著性(P<0.01)。结论:建立大鼠慢性高眼压模型操作简单,重复性好,成模率高,高眼压可稳定维持长时间,慢性高眼压将导致整个视网膜的变薄和萎缩。

Construction and appraisal of chronic ocular hypertension glaucoma model of rats

AIM:The establishment of chronic ocular hypertension surgery glaucoma animal model for the study of the pathogenesis,treatment and protection of these three aspects of the optic nerve is particularly necessary.·METHODS: Superficial burning three episcleral veins,and surgery combined with conjunctival flap under the Baptist has the mitomycin C 0.2g/L gel sponge ,to block rat aqueous outflow of the eye can induce by intraocular pressure(IOP),the establishment of rat model of chronic high intraocular pressure. After surgery 2 hours,1 day,3,7,14,28,56 days,5 rats were killed,blank control group,also killed 5 rats;Eyeballs were removed for light microscopy,retinal eye patterns observation and measurement of retinal thickness in order to study the IOP,retinal ganglion cells( RGC)and optic nerve injury characteristics. ·RESULTS: The rats with sham surgery eye eyes,eye control of postoperative intraocular pressure at different time points of comparison there was a significant difference (P<0.01). ·CONCLUSION: Rats with chronic ocular hypertension model is simple,reproducible and high rate into a mold,high intraocular pressure may maintain stability for a long time.Chronic high intraocular pressure of the final results will lead to the thinning of the retina and atrophy.