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生物法和HPLC测定左氧氟沙星血药浓度的比较
引用本文:司凯英,段京莉,严宝霞. 生物法和HPLC测定左氧氟沙星血药浓度的比较[J]. 中国药学杂志, 1999, 0(1): 43-45
作者姓名:司凯英  段京莉  严宝霞
作者单位:1. 大庆163001黑龙江大庆油田总医院药剂科
2. 北京100083北京医科大学第三医院药剂科
摘    要: 目的:建立用生物法和HPLC测定左氧氟沙星血药浓度的方法,为临床合理用药提供依据。方法:生物法使用培养基Ⅱ号和金葡球菌。HPLC用300g·L-1三氯醋酸沉淀蛋白,流动相为甲醇0.02mol·L-1-磷酸二氢钾溶液(3∶7,1mol·L-1磷酸调节pH=3.0),检验波长290nm.结果:生物法测定不同浓度左氧氟沙星的平均回收率在95.90%~106.68%范围内,日内和日间RSD低于6%。HPLC测定不同浓度左氧氟沙星的平均回收率在97.24%~101.70%范围内,日内和日间RSD低于5%。6例患者以两种方式po左氧氟沙星片剂后,用生物法和HPLC测其血药浓度,结果经方差分析及t检验分析,无显著性差异(P>0.05)。结论:生物法价廉、省时、结果准确可靠;HPLC灵敏度高、分析速度快、分离效果好。这两种方法可以根据具体的实验条件选用。

关 键 词:左氧氟沙星  生物法  HPLC
收稿时间:1977-10-28;

Comparison of bioassay and HPLC for the determination of levofloxacin in plasma
Si Kaiying,Duan Jingli,Yan Baoxia. Comparison of bioassay and HPLC for the determination of levofloxacin in plasma[J]. Chinese Pharmaceutical Journal, 1999, 0(1): 43-45
Authors:Si Kaiying  Duan Jingli  Yan Baoxia
Affiliation:Si Kaiying (Si KY),Duan Jingli(Duan JL),Yan Baoxia (Yan BX) (
Abstract:OBJECTIVE:The bioassay and HPLC methods were established and compared for the determination of levofloxacin(Lev) in human plasma.METHOD:In bioassay,medium Ⅱ and staphylococcus aureus were used.The HPLC method was chromatographed with a mobile phase of methanol-0.02mol/L potassium dihydrogen phosphate solution (3∶7),the sample was treated by adding 30% trichloroacetic acid to precipitate protein,and detected at 276mm.RESULTS:The average recovery of bioassay was within 95.90%~106.68%,the within-day and day-to-day precisions were below 6%.The average recovery of HPLC was within 97.24%~101.70%,the within day and day-to-day precisions were blow 5%.Lev tablets were given to 6 patients in two ways.Drug concentrations in plasma were determined by bioassay and HPLC assay,respectively,with no significant difference (P>0.05).CONCLUSION:The bioassay was simple,cheap,time saving,and reliable.The HPLC method wsa rapid,sensitive,accurate.The two methods may be selected to use in actual conditions.
Keywords:levofloxacin  bioassay  HPLC
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