Initial concentration of Staphylococcus epidermidis in simulated pediatric blood cultures correlates with time to positive results with the automated, continuously monitored BACTEC blood culture system |
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Authors: | Haimi-Cohen Yishai Vellozzi Ernestine M Rubin Lorry G |
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Affiliation: | Department of Pediatrics, Schneider Children's Hospital, Long Island Jewish Medical Center of the North Shore-Long Island Jewish Health System, New Hyde Park, New York, USA. |
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Abstract: | The relationship of initial concentration of Staphylococcus epidermidis in blood cultures and time to positivity (TTP) in an automated, continuously monitored blood culture system was assessed. Blood and 1 to 1,000 CFU of S. epidermidis per ml in stationary or exponential phase were inoculated in BACTEC Pediatric Plus F bottles and incubated. The TTP was inversely proportional to the initial inoculated concentration. Blood culture bottles with initial bacterial densities of <10 CFU/ml had a TTP of >20 h (upper limit of 95% prediction interval, 20.7 h) and bottles with initial bacterial densities of > or =50 CFU/ml had a TTP of < or =15 h (lower limit of 95% prediction interval, 13.4 h). |
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