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Analysis of T lymphocytes cloned from the synovial fluid and blood of a patient with lyme arthritis |
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| Authors: | Yssel, Hans Nakamoto, Tetsuya Schneider, Patricia Freltas, Vickl Collins, Carol Webb, David Mensi, Nahla Soderberg, Carol Peltz, Gary |
| Affiliation: | Department of Human Immunology, DNAX Research Institute Palo Alto, CA, USA 1 Institutes of Cancer and Developmental Biology, SyntexResearch Palo Alto, CA, USA 2 Institutes of Biological Science, SyntexResearch Palo Alto, CA, USA 3 Institutes of Molecular Immunology, SyntexResearch Palo Alto, CA, USA |
| Abstract: | Cloned T lymphocytes reactive with Borrella burgdorferi proteinswere isolated from a patient with chronic Lyme arthritis. Allof the T cell clones which proliferated In response to Borrellaproteins were CD3+CD4+CD8-TCR ß+ and HLA-DR restricted.One T cell clone (GN30) exhibited HLA-DR-restricted cytotoxicactivity against antigen-presenting cells pulsed with Borrellaantigen. In response to Borrella antigen, the T cell clonesproduced TNF-, INF-ß, and GM-CSF. There are at leastthree distinct spirochetal proteins recognized by the four Tcell clones analyzed. Purified Borrella proteins triggered theHLA-DR-restrlcted proliferative and cytotoxic responses, aswell as lymphokine secretion by two of the T cell clones. Thespirochetal protein which triggered the HLA-DR-restrlcted proliferativeand cytotoxic activities of the T cell clone (GN30) isolatedfrom synovial fluid is the 41 kd flagellar protein. |
| Keywords: | Lyme disease T cells inflammary arthritis |
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