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| 云南榧子中多糖的含量测定及其高效毛细管电泳指纹图谱分析 | |
| 引用本文： | 陈振德,谢立,何英,梁强.云南榧子中多糖的含量测定及其高效毛细管电泳指纹图谱分析[J].中国药房,2005,16(23):1817-1818. |
| 作者姓名： | 陈振德 谢立 何英 梁强 |
| 作者单位： | 1. 深圳市人民医院药学部,深圳市,518020 2. 广州市胸科医院药剂科,广州市,510095 3. 深圳市第二人民医院药剂科,深圳市,518035 |
| 基金项目： | 中国科学院资助项目，广东省博士启动基金，广东省深圳市科技计划 |
| 摘 要： | 目的:建立以苯酚-硫酸比色法测定云南榧子中多糖含量的方法。方法:检测波长为490nm,狭缝宽度为2nm,记录纸范围为20nm/cm;采用高效毛细管电泳法分析不同产地的云南榧子。结果:无水葡萄糖检测量在0·0114mg~0·1482mg范围内与吸收度线性关系良好(r=0·9994),平均加样回收率为98·3%(RSD=2·31%,n=5);不同产地云南榧子高效毛细管电泳指纹图谱基本一致,且主要含2种多糖。结论:不同产地云南榧子中多糖含量及组成无明显差异。 |
| 关 键 词： | 云南榧子 多糖 比色法 含量 高效毛细管电泳法 指纹图谱 |
| 文章编号： | 1001-0408（2005）23-1817-02 |
| 收稿时间： | 2005-02-20 |
| 修稿时间： | 2005-05-12 |
Assaying of Polysaccharides in Torreya Yunnanensis and Analysis of HPCE Fingerprints |
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| CHEN Zhende,XIE Li,HE Ying,LIANG Qiang.Assaying of Polysaccharides in Torreya Yunnanensis and Analysis of HPCE Fingerprints[J].China Pharmacy,2005,16(23):1817-1818. | |
| Authors: | CHEN Zhende XIE Li HE Ying LIANG Qiang |
| Abstract: | OBJECTIVE:To establish phenol-vitriolic colorimetry for the assaying of polysaccharides in Torreya yunnanensis.METHODS:Detection wavelength was 490nm,slit-width was 2nm and record paper range was 20nm/cm;Torreya yun_nanensis from different origins were analyzed by HPCE.RESULTS:The linear range of anhydrous dextrose was 0.0 114mg～0.1 482mg(r=0.9 994);The average recovery rate was 98.3%(RSD=2.31%,n=5).The HPCE fingerprints of Torreya yu_nnanensis from different origins were basically the same,and 2 kinds of polysaccharides were contained in whcih.CONCLUSI_ON:The contents and compositions of polysaccharides of Torreya yunnanensis from different origins were of no significant diff_erence. |
| Keywords: | Torreya yunnanensis Polysaccharides Colorimetry Content HPCE Fingerprint |
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