PTEN、PI3K和Akt蛋白在胃癌组织中的表达及其临床意义

目的:探讨PTEN、PI3K和Akt在胃癌中的蛋白表达及其与胃癌临床病理特征的关系,以及PTEN、PI3K和Akt三者之间的相关性。方法:采用免疫组织化学Elivision法检测68例胃癌组织和10例正常胃黏膜组织中PTEN、PI3K和Akt的蛋白表达。结果:胃癌组织中PTEN、PI3K、Akt蛋白的阳性表达率分别为48.53%(33/68)、85.29%(58/68)和89.71%(61/68)。早期胃癌、中晚期胃癌中PTEN、PI3K、Akt蛋白的阳性表达率与正常胃黏膜组织相比,差异均有显著性(P<0.05)。PTEN、PI3K、Akt蛋白的表达均与胃癌癌细胞的分化程度、淋巴结转移显著相关(P<0.05),且PTEN蛋白表达与胃癌Lauren分型显著相关(P<0.05),但PI3K、Akt蛋白表达与胃癌的Lauren分型无关(P>0.05)。在68例胃癌组织中,PTEN分别与PI3K、Akt的蛋白表达之间均呈显著负相关(P<0.05),PI3K与Akt蛋白表达呈显著正相关(P=0.001)。结论:PTEN、PI3K、Akt蛋白的表达均与胃癌发生发展及侵袭转移有关,但PTEN与弥漫型胃癌发生发展的关系更加密切。在胃癌的发生发展中PI3K、PTEN的作用可能互相拮抗;PTEN蛋白的表达缺失可能与Akt过表达有关;PI3K和Akt协同促进胃癌的恶性进展。

Expressions of PTEN, PI3K and Akt Protein in Gastric Carcinoma and Their Clinical Significance

Objective: To investigate the expressions of PTEN,PI3K and Akt proteins in gastric carcinoma and their relationship between their expressions and clinical pathology parameters,and correlation among PTEN,PI3K and Akt protein expression each other.Methods: Elivision immunohistochemical method was used to detect expressions of PTEN,PI3K and Akt proteins in ten normal gastric mucosa tissues and 68 gastric carcinoma tissues.Results: In 68 gastric carcinoma tissues,PTEN,PI3K and Akt proteins positive rates was 48.53%(33/68),85.29%(58/68) and 89.71%(61/68) respectively.There were significant differences of PTEN,PI3K and Akt proteins expressions between gastric carcinoma and normal gastric mucosa epithelium(all P<(0.05).) There were significant correlation between PTEN,PI3K and Akt proteins expression and lymph node metastasis or cell differentiation degree respectively(all P<0.05).Moreover,significant correlation was detected between positive rates of PTEN protein and Lauren type of gastric carcinoma(P=0.022).In 68 cases of gastric carcinoma,a negative correlation was found between the expression of PTEN and PI3K or Akt proteins respectively(both P<0.05),and there was a highly positive correlation between PI3K and Akt protein expression(P=0.001).Conclusion: All of PTEN,PI3K and Akt proteins expressions were related with the development,progress,invasiveness and metastasis of gastric carcinoma,and PTEN was related closely with the development of diffuse-type gastric carcinoma.In the development and progress of gastric carcinoma,PI3K and PTEN may counteract each other,and the loss of PTEN protein expression may be related with the overexpression of Akt protein.PI3K and Akt protein may cooperate to promote malignant progress of gastric carcinoma.