DDR1促进胰腺癌细胞AsPC-1的迁移及侵袭能力

目的 探讨盘状结构域受体1 (DDR1)对胰腺癌细胞迁移及侵袭能力的影响.方法 利用qRT-PCR检测DDR1在胰腺癌旁组织及癌组织中的表达水平.通过脂质体转染DDR1表达质粒至胰腺癌细胞AsPC-1中,应用Western blot验证其转染效果.通过划痕实验及Transwell法检测转染DDR1表达质粒后细胞迁移及侵袭能力的变化情况.Western blot检测转染后基质金属蛋白酶2 (MMP2)和基质金属蛋白酶9 (MMP9)的表达水平.结果 与癌旁组织比较,胰腺癌组织的DDR1 mRNA表达水平显著升高(P<0.05).转染质粒后,AsPC-1细胞DDR1蛋白表达量显著升高(P<0.05).应用划痕试验及Transwell试验结果显示,与对照组比较,AsPC-1/DDR1迁移力上调(51.11±11.51)%,侵袭力上调(77.25±10.64)%,差异均具有统计学意义(P<0.05).过表达DDR1后,AsPC-1细胞中MMP2和MMP9表达水平显著升高(P<0.05).结论 DDR1通过改变MMP2和MMP9的表达水平,促进胰腺癌细胞的迁移及侵袭,有望成为靶向治疗的新方向.

Up-regulating effect of DDR1 on migration and invasion of human pancreatic cancer cell line AsPC-1

Objective To investigate the effect of discoidin domain receptor 1 (DDR1) on the migration and in-vasion of human pancreatic cancer cell line AsPC-1. Methods The expressions of DDR1 mRNA in pancreatic cancer tissues and adjacent tissues were determined by real-time PCR. The expressions of DDR1, matrix metalloproteinase 2 (MMP2) and matrix metalloproteinase 2 (MMP9) were detected by Western blot after the DDR1 expression plasmid was transfected into AsPC-1 cell line. The ability of migration and invasion was determined by Wound Healing and Tran-swell. Results The expression of DDR1 mRNA in pancreatic cancer tissues was significantly higher than that in adja-cent tissues (P<0.05). After the DDR1 plasmid was transfected into AsPC-1, the protein expression of DDR1 was signifi-cantly up-regulated (P<0.05). Compared with cells transfected with empty vector (control), the ability of migration and invasion for cells transfected with DDR1 plasmid were increased by (51.11 ± 11.51)%and (77.25 ± 10.64)%, and the ex-pression levels of MMP2 and MMP9 after overexpression of DDR1 were also significantly up-regulated. The differences were all statistically significant (P<0.05). Conclusion DDR1 can promote the migration and invasion of human pancre-atic caner cell line AsPC-1 via up-regulating the expressions of MMP2 and MMP9. It could be considered as a potential target for gene therapy for pancreatic cancer.