双下肢骨折大鼠血液粘度及血清酶活性变化

目的:观察骨折对大鼠血液流变学及血清酶学的影响。方法:20只Wistar大鼠,分为对照组、骨折组。观察双下肢骨折后血液流变学指标以及血清AST、ALT、LDH、LDH-1、HBDH、CK、CK-MB、ALP等指标的变化。结果:骨折组大鼠高、中、低切变率下全血粘度及血浆粘度均显著高于对照组,且血清LDH-1、CK-MB、ALP活性显著高于对照组(P<0.01),而血清AST、ALT、LDA、HBDA、CK活性与对照组无统计学差异(P>0.05)。结论:骨折可致大鼠血液流变性异常以及某些血清酶释放增多,从而引起器官功能障碍。

Changes of Blood Viscosity and Serum Enzyme Activity in Rats with Double Crura Fracture

Objective: To study the effect of fracture on hemorheology and serum enzymology in rats.Method: 20 Wistar rats were divided into two groups: fracture group and control group. Observe the changes of hemorheological indexes and serum enzymology,including aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactic acid dehydrogenase (LDH), LDH-1, β-hydroxybutyrate dehydrogenase (HBDH), creatine phosphokinase (CK), CK-MB and alkaline phosphatase (ALP). Results: The high, middle and low shear of blood viscosity and plasma viscosity in fracture group were significantly higher than that of the control group; and the serum activities of LDH-1, CK-MB and ALP in serum in fracture group were significantly higher than that of the control group (P<0.01), while the activities of AST, ALT, LDA, HBDA and CK expressed no statistical differences between fracture group and control group (P>0.05). Conclusion: The results suggested that fracture could induce abnormal hemorheological properties and increased serum enzyme release,resulting in the disturbance of organic function.