分光光度法测定蛇床子中铜、铁、锌含量的研究

目的:探讨分光光度法测定蛇床子中金属元素含量的可行性,并阐述蛇床子的药理功效与金属元素种类、含量可能存在的一定关系。方法:根据不同的金属离子与特定显色剂显色,产生可见光吸收,采用分光光度法平行测定人发标准品及蛇床子中金属元素的含量。结果:所选的蛇床子中均含丰富的微量金属元素,其中铁元素含量较其它元素高,高达136.69 mg/kg,锌元素为51.72 mg/kg,铜元素为33.49 mg/kg。结论:分光光度法操作简便、测量快速、结果准确,易推广使用。蛇床子的药理功效与其中的金属元素种类及含量存在密切的联系,有待进一步深入研究。

Determination of copper,iron and zinc in Fructus cnidii by spectrophotometry

Objective:To discuss the feasibility of determining the contents of trace element in Fructus cnidii by spectrophotometry,and to expound the potential relationship between the sorts and contents of the elements in crude drugs and the pharmacological function of Fructus cnidii.Methods:Different metal ions can be colored with specific color reagent,engendering visible light absorption,so spectrophotometry was adopted to determine the contents both of the Fructus cnidii and the standard substance of human hair.Results:The results showed that the Fructus cnidii was rich in trace element,the content of Fe was especially higher.Fe in Fructus cnidii is highest to 136.69mg/kg,Zn is highest to 51.72mg/kg,Cu is highest to 33.49mg/kg.Conclusion:Spectrophotometry was simple,high-speed and accurate,which could be popularized widely.The relationship between the sorts and contents of the elements in Fructus cnidii and the pharmacological function of them is so consanguineous that the further research is needed to ascertain.